Publications of Sascha Young Kupke

Pelz, L., Dogra, T., Marichal-Gallardo, P., Hein, M. D., Hemissi, G., Kupke, S. Y., Genzel, Y., & Reichl, U. (2024)Production of antiviral “OP7 chimera” defective interfering particles free of infectious virusApplied Microbiology and Biotechnology, 108, 1–15doi: 10.1007/s00253-023-12959-6.

Rüdiger, D., Piasecka, J., Küchler, J., Pontes, C., Laske, T., Kupke, S. Y., & Reichl, U. (2024)Mathematical model calibrated to in vitro data predicts mechanisms of antiviral action of the influenza defective interfering particle “OP7”iScience, 27 (4), 109421doi: 10.1016/j.isci.2024.109421.

Dogra, T., Pelz, L., Boehme, J. D., Küchler, J., Kershaw, O., Marichal-Gallardo, P., Baelkner, M., Hein, M. D., Gruber, A. D., Benndorf, D., Genzel, Y., Bruder, D., Kupke, S. Y., & Reichl, U. (2023)Generation of “OP7 chimera” defective interfering influenza A particle preparations free of infectious virus that show antiviral efficacy in miceScientific Reports, 13, 20936doi: 10.1038/s41598-023-47547-1.

Hein, M. D., Kazenmaier, D., van Heuvel, Y., Dogra, T., Cattaneo, M., Kupke, S. Y., Stitz, J., Genzel, Y., & Reichl, U. (2023)Production of retroviral vectors in continuous high cell density cultureApplied Microbiology and Biotechnology, 107, 5947–5961doi: 10.1007/s00253-023-12689-9.

Pelz, L., Piagnani, E., Marsall, P., Wynserski, N., Hein, M. D., Marichal-Gallardo, P., Kupke, S. Y., & Reichl, U. (2023)Broad-Spectrum Antiviral Activity of Influenza A Defective Interfering Particles against Respiratory Syncytial, Yellow Fever, and Zika Virus Replication In VitroViruses, 15 (9), 1872doi: 10.3390/v15091872.

Küchler, J., Püttker, S., Lahmann, P., Genzel, Y., Kupke, S. Y., Benndorf, D., & Reichl, U. (2022)Absolute quantification of viral proteins during single-round replication of MDCK suspension cellsJournal of Proteomics, 259, 104544doi: 10.1016/j.jprot.2022.104544.

Hein, M. D., Arora, P., Marichal-Gallardo, P., Winkler, M., Genzel, Y., Pöhlmann, S., Schughart, K., Kupke, S. Y., & Reichl, U. (2021)Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particlesBMC Biology, 19 (1), 91doi: 10.1186/s12915-021-01020-5.

Hein, M. D., Chawla, A., Cattaneo, M., Kupke, S. Y., Genzel, Y., & Reichl, U. (2021)Cell culture–based production of defective interfering influenza A virus particles in perfusion mode using an alternating tangential flow filtration systemApplied Microbiology and Biotechnology, 105, 7251–7264doi: 10.1007/s00253-021-11561-y.

Hein, M. D., Kollmus, H., Marichal-Gallardo, P., Püttker, S., Benndorf, D., Genzel, Y., Schughart, K., Kupke, S. Y., & Reichl, U. (2021)OP7, a novel influenza A virus defective interfering particle: production, purification, and animal experiments demonstrating antiviral potentialApplied Microbiology and Biotechnology, 105 (1), 129–146doi: 10.1007/s00253-020-11029-5.

Pelz, L., Rüdiger, D., Dogra, T., Alnaji, F. G., Genzel, Y., Brooke, C. B., Kupke, S. Y., & Reichl, U. (2021)Semi-continuous propagation of influenza A virus and its defective interfering particles: analyzing the dynamic competition to select candidates for antiviral therapyJournal of Virology, 95 (24), e01174-21doi: 10.1128/JVI.01174-21.

Rand, U., Kupke, S. Y., Shkarlet, H., Hein, M. D., Hirsch, T., Marichal-Gallardo, P., Cicin-Sain, L., Reichl, U., & Bruder, D. (2021)Antiviral Activity of Influenza A Virus Defective Interfering Particles against SARS-CoV-2 Replication In Vitro through Stimulation of Innate ImmunityCells, 10 (7), 1756doi: 10.3390/cells10071756.

Rüdiger, D., Pelz, L., Hein, M. D., Kupke, S. Y., & Reichl, U. (2021)Multiscale model of defective interfering particle replication for influenza A virus infection in animal cell culturePLoS Computational Biology, 17, e1009357doi: 10.1371/journal.pcbi.1009357.

Kupke, S. Y., Ly, L.-H., Börno, S. T., Ruff, A., Timmermann, B., Vingron, M., Haas , S., & Reichl, U. (2020)Single-Cell Analysis Uncovers a Vast Diversity in Intracellular Viral Defective Interfering RNA Content Affecting the Large Cell-to-Cell Heterogeneity in Influenza A Virus ReplicationViruses, 12 (1), 71doi: 10.3390/v12010071.

Kupke, S. Y., Riedel, D., Frensing, T., Zmora, P., & Reichl, U. (2019)A Novel Type of Influenza A Virus-Derived Defective Interfering Particle with Nucleotide Substitutions in Its GenomeJournal of Virology, 93 (4), 01786-18doi: 10.1128/JVI.01786-18.

Rüdiger, D., Kupke, S. Y., Laske, T., Zmora, P., & Reichl, U. (2019)Multiscale modeling of influenza A virus replication in cell cultures predicts infection dynamics for highly different infection conditionsPLoS Computational Biology, 15 (2), e1006819doi: 10.1371/journal.pcbi.1006819.

Frensing, T., Kupke, S. Y., Bachmann, M., Fritzsche, S., Gallo Ramirez, L. E., & Reichl, U. (2016)Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cellsApplied Microbiology and Biotechnology, 100 (16), 7181–7192doi: 10.1007/s00253-016-7542-4.

Heldt, F. S., Kupke, S. Y., Dorl, S., Frensing, T., & Reichl, U. (2015)Single-cell analysis and stochastic modelling unveil large cell-to-cell variability in influenza A virus infectionNature Communications, 6, 8938doi: 10.1038/ncomms9938.

Rüdiger, D., Kupke, S. Y., & Reichl, U. (2018)A multiscale model of influenza A virus replication covering highly different infection conditions in cell culturesIn: IFAC-PapersOnLine, 32–33doi: 10.1016/j.ifacol.2018.09.026.

Hein, M., Tapia, F., Genzel, Y., Kupke, S. Y., & Reichl, U. (2019)Continuous mode of production for two classes of defective interfering influenza A virus particles as antiviral candidatesIn: Integrated Continuous Biomanufacturing IV, 76.

Kupke, S. Y., Riedel, D., Frensing, T., Zmora, P., & Reichl, U. (2018)Single-cell analysis uncovers a novel influenza A virus-derived defective interfering particle for antiviral therapyIn: Vaccine Technology VII, 133.