Publications of Pavel Marichal-Gallardo

Offersgaard, A., Duarte Hernandez, C. R., Zhou, Y., Duan, Z., Gammeltoft, K. A., Hartmann, K. T., Fahnøe, U., Marichal-Gallardo, P., Alzua, G. P., Underwood, A. P., Sølund, C., Weis, N., Bonde, J. H., Christensen, J. P., Pedersen, G. K., Jensen, H. E., Holmbeck, K., Bukh, J., & Gottwein, J. M. (2024)An inactivated SARS-CoV-2 vaccine based on a Vero cell culture-adapted high-titer virus confers cross-protection in small animalsScientific Reports, 14 (1), 17039doi: 10.1038/s41598-024-67570-0.

Pelz, L., Dogra, T., Marichal-Gallardo, P., Hein, M. D., Hemissi, G., Kupke, S. Y., Genzel, Y., & Reichl, U. (2024)Production of antiviral “OP7 chimera” defective interfering particles free of infectious virusApplied Microbiology and Biotechnology, 108, 97doi: 10.1007/s00253-023-12959-6.

Dogra, T., Pelz, L., Boehme, J. D., Küchler, J., Kershaw, O., Marichal-Gallardo, P., Baelkner, M., Hein, M. D., Gruber, A. D., Benndorf, D., Genzel, Y., Bruder, D., Kupke, S. Y., & Reichl, U. (2023)Generation of “OP7 chimera” defective interfering influenza A particle preparations free of infectious virus that show antiviral efficacy in miceScientific Reports, 13, 20936doi: 10.1038/s41598-023-47547-1.

Offersgaard, A., Duarte Hernandez, C. R., Feng, S., Marichal-Gallardo, P., Holmbeck, K., Pihl, A. F., Fernandez-Antunez, C., Peña Alzua, G., Top Hartmann, K., Pham, L. V., Zhou, Y., Anbro Gammeltoft, K., Fahnøe, U., Schneider, U. V., Pedersen, G. K., Jensen, H. E., Pravsgaard Christensen, J., Ramirez, S., Bukh, J., & Gottwein, J. M. (2023)An inactivated SARS-CoV-2 vaccine induced cross-neutralizing persisting antibodies and protected against challenge in small animalsiScience, 26 (2), 105949doi: 10.1016/j.isci.2023.105949.

Pelz, L., Piagnani, E., Marsall, P., Wynserski, N., Hein, M. D., Marichal-Gallardo, P., Kupke, S. Y., & Reichl, U. (2023)Broad-Spectrum Antiviral Activity of Influenza A Defective Interfering Particles against Respiratory Syncytial, Yellow Fever, and Zika Virus Replication In VitroViruses, 15 (9), 1872doi: 10.3390/v15091872.

Bissinger, T., Wu, Y., Marichal-Gallardo, P., Riedel, D., Liu, X., Genzel, Y., Tan, W. S., & Reichl, U. (2021)Towards integrated production of an influenza A vaccine candidate with MDCK suspension cellsBiotechnology and Bioengineering, 118 (10), 3996–4013doi: 10.1002/bit.27876.

Gränicher, G., Babakhani, M., Göbel, S., Jordan, I., Marichal-Gallardo, P., Genzel, Y., & Reichl, U. (2021)A high cell density perfusion process for Modified Vaccinia virus Ankara production: process integration with inline DNA digestion and cost analysisBiotechnology and Bioengineering, 118 (12), 4720–4734doi: 10.1002/bit.27937.

Hein, M. D., Arora, P., Marichal-Gallardo, P., Winkler, M., Genzel, Y., Pöhlmann, S., Schughart, K., Kupke, S. Y., & Reichl, U. (2021)Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particlesBMC Biology, 19 (1), 91doi: 10.1186/s12915-021-01020-5.

Hein, M. D., Kollmus, H., Marichal-Gallardo, P., Püttker, S., Benndorf, D., Genzel, Y., Schughart, K., Kupke, S. Y., & Reichl, U. (2021)OP7, a novel influenza A virus defective interfering particle: production, purification, and animal experiments demonstrating antiviral potentialApplied Microbiology and Biotechnology, 105 (1), 129–146doi: 10.1007/s00253-020-11029-5.

Mahour, R., Marichal-Gallardo, P., Rexer, T., & Reichl, U. (2021)Multi-enzyme cascades for the in vitro synthesis of guanosine diphosphate L-fucoseChemCatChem, 13 (8), 1981–1989doi: 10.1002/cctc.202001854.

Marichal-Gallardo, P., Börner, K., Pieler, M., Sonntag-Buck, V., Obr, M., Bejarano, D., Wolff, M. W., Kräusslich, H.-G., Reichl, U., & Grimm, D. (2021)Single-use capture purification of adeno-associated viral gene transfer vectors by membrane-based steric exclusion chromatographyHuman Gene Therapy, 32 (17-18), 959–974doi: 10.1089/hum.2019.284 .

Rand, U., Kupke, S. Y., Shkarlet, H., Hein, M. D., Hirsch, T., Marichal-Gallardo, P., Cicin-Sain, L., Reichl, U., & Bruder, D. (2021)Antiviral Activity of Influenza A Virus Defective Interfering Particles against SARS-CoV-2 Replication In Vitro through Stimulation of Innate ImmunityCells, 10 (7), 1756doi: 10.3390/cells10071756.

Gränicher, G., Coronel, J., Pralow, A., Marichal-Gallardo, P., Wolff, M. W., Rapp, E., Karlas, A., Sandig, V., Genzel, Y., & Reichl, U. (2019)Efficient influenza A virus production in high cell density using the novel porcine suspension cell line PBG.PK2.1Vaccine, 37 (47), 7019–7028doi: 10.1016/j.vaccine.2019.04.030.

Marichal-Gallardo, P., Pieler, M., Wolff, M. W., & Reichl, U. (2017)Steric exclusion chromatography for purification of cell culture-derived influenza A virus using regenerated cellulose membranes and polyethylene glycolJournal of Chromatography A, 1483 (3), 110–119doi: 10.1016/j.chroma.2016.12.076.

Marichal-Gallardo, P., Genzel, Y., Börner, K., Grimm, D., Wolff, M. W., & Reichl, U. (2019)A single-use chromatographic purification platform for viral gene transfer vectors & viral vaccinesIn: Proceedings Advancing Manufacture of Cell and Gene Therapies VI, 92.

Marichal-Gallardo, P., Fortuna, A. R., Genzel, Y., Wolff, M. W., & Reichl, U. (2018)Purifying viruses with a sheet of paper: Single-use steric exclusion chromatography as a capture platform for vaccine candidatesIn: Vaccine Technology VII, 128.

Pelz, L., Rüdiger, D., Hein, M., Böhme, J., Bälkner, M., Kershaw, O., Marichal-Gallardo, P., Gruber, A., Genzel, Y., Bruder, D., Kupke, S. Y., & Reichl, U.Engineered defective interfering particles of influenza A virus for antiviral treatmentPresented at: 33^rd Annual Meeting of the Society for Virology (GfV)Vienna (Austria), March 25, 2023.

Pelz, L., Rüdiger, D., Hein, M. D., Böhme, J., Bälkner, M., Kershaw, O., Marichal-Gallardo, P., Gruber, A., Genzel, Y., Bruder, D., Kupke, S. Y., & Reichl, U.Genetic engineering of Influenza A virus defective interfering particles towards improved antiviral efficacy and potential use as a live vaccinePresented at: Vaccine Technology IXLos Cabos (Mexico), May 19, 2024.

Dogra, T., Pelz, L., Boehme, J. D., Baelkner, M., Kershaw, O., Marichal-Gallardo, P., Gruber, A. D., Genzel, Y., Bruder, D., Kupke, S. Y., & Reichl, U.Genetically engineered defective interfering particles of influenza A virus for antiviral treatment and vaccinationPresented at: 17^th Vaccine CongressGlasgow (Scotland), September 24, 2023.

Pelz, L., Dogra, T., Boehme, J. D., Hein, M. D., Marichal-Gallardo, P., Genzel, Y., Bruder, D., Kupke, S. Y., & Reichl, U.Influenza A virus OP7 defective interfering particles: Cell culture-based production and antiviral efficacy in vivoPresented at: Cell Culture Engineering (CCE) XVIII Cancun (Mexico), April 23, 2023.