Publications of Michael Pieler

Marichal-Gallardo, P., Börner, K., Pieler, M., Sonntag-Buck, V., Obr, M., Bejarano, D., Wolff, M. W., Kräusslich, H.-G., Reichl, U., & Grimm, D. (2021)Single-use capture purification of adeno-associated viral gene transfer vectors by membrane-based steric exclusion chromatographyHuman Gene Therapy, 32 (17-18), 959–974doi: 10.1089/hum.2019.284 .

Hämmerling, F., Pieler, M., Hennig, R., Serve, A., Rapp, E., Wolff, M. W., Reichl, U., & Hubbuch, J. (2017)Influence of the production system on the surface properties of influenza A virus particlesEngineering in Life Sciences, 17 (10), 1071–1077doi: 10.1002/elsc.201700058.

Marichal-Gallardo, P., Pieler, M., Wolff, M. W., & Reichl, U. (2017)Steric exclusion chromatography for purification of cell culture-derived influenza A virus using regenerated cellulose membranes and polyethylene glycolJournal of Chromatography A, 1483 (3), 110–119doi: 10.1016/j.chroma.2016.12.076.

Pieler, M., Heyse, A., Wolff, M. W., & Reichl, U. (2017)Specific ion effects on the particle size distributions of cell culture–derived influenza A virus particles within the Hofmeister seriesEngineering in Life Sciences, 17 (5), 470–478doi: 10.1002/elsc.201600153.

Pieler, M., Frentzel, S., Bruder , D., Wolff, M. W., & Reichl, U. (2016)A cell culture-derived whole virus influenza A vaccine based on magnetic sulfated cellulose particles confers protection in mice against lethal influenza A virus infectionVaccine, 34 (50), 6367–6374doi: 10.1016/j.vaccine.2016.10.041.

Serve, A., Pieler, M., Benndorf, D., Rapp, E., Wolff, M. W., & Reichl, U. (2015)Comparison of Influenza Virus Particle Purification Using Magnetic Sulfated Cellulose Particles with an Established Centrifugation Method for AnalyticsAnalytical Chemistry, 87 (21), 10708–10711doi: 10.1021/acs.analchem.5b02681.

Pieler, M., Frentzel, S., Bruder , D., Wolff, M. W., & Reichl, U. (2016)Pseudo-affinity purification and formulation of a cell-culture derived whole influenza virus vaccine using magnetic sulfated cellulose particlesIn: Vaccine Technology VI, 57.

Marichal-Gallardo, P., Pieler, M., Boerner, K., Riedel, D., Wolff, M. W., Reichl, U., & Grimm, D.Disposable membrane adsorbers for size-based chromatographic purification of virus particles & gene therapy vectorsPresented at: ÄKTA User Day 2016Berlin, Germany, September 26, 2016.

Marichal-Gallardo, P., Pieler, M., Riedel, D., Wolff, M. W., & Reichl, U.Purification of cell culture-based influenza A virus particles with a cellulose membrane adsorber and polyethylene glycolPresented at: International Symposium on the Separation of Proteins, Peptides, and Polynucleotides (ISPPP) 2016Salzburg, Austria, November 6, 2016.

Marichal-Gallardo, P., Pieler, M., Wolff, M. W., & Reichl, U.Purification of cell culture-based influenza virus particles using membrane filters with the aid of non-ionic polymersPresented at: PREP: Preparative and Process Chromatography Symposium 2016Philadelphia, USA, July 17, 2016.

Pieler, M., Hennig, R., Gebert, N., Rapp, E., Wolff, M. W., & Reichl, U.Comparison of protein and glyco patterns of cell culture-derived influenza viruses purified by pseudo affinity ligandsPresented at: 12th International PhD Seminar on Chromatographic Separation ScienceSchleiden, February 13, 2016.

Pieler, M., Serve, A., Hennig, R., Vazquez-Ramirez, D., Wolff, M. W., & Reichl, U.Virus Particle Purification Using Magnetic Sulfated Cellulose ParticlesPresented at: Himmelfahrtstagung: New Frontiers for Biotech-Processes Rhein-Mosel-Halle Koblenz/Germany , May 2, 2016.

Wolff, M. W., Fortuna, A. R., Pieler, M., Weigel, T., Fischer, L. M., & Reichl, U.Upcoming chromatography media for cell culture-derived influenza virus purificationPresented at: ProcessNetJahrestagung und Jahrestagung der Biotechnologen 2014 Aachen, Germany, September 30, 2014.

Marichal-Gallardo, P., Pieler, M., Riedel, D., Wolff, M. W., & Reichl, U.Chromatographic purification of cell culture-derived influenza A virus particles using cellulose filters and polyethylene glycolPresented at: International Symposium on Preparative and Industrial Chromatography and Allied Techniques (SPICA) 2016 Vienna, Austria, October 9, 2016.

Pieler, M., Frentzel, S., Bruder, D., Wolff, M. W., & Reichl, U.Pseudo-affinity purification and formulation of a cell culture-derived whole influenza virus vaccine using magnetic sulfated cellulose particlesPresented at: Vaccine Technology Albufeira 2016Albufeira, Portugal, June 12, 2016.

Vazquez-Ramirez, D., Lemke, V., Pieler, M., Genzel, Y., Jordan, I., Sandig, V., & Reichl, U.Process optimization for semi-continuous virus production at high cell densitiesPresented at: Cell Culture Engineering XVPalm Springs, CA, USA, May 8, 2016.

Wolff, M. W., Pieler, M., Frentzel, S., Fichtmüller, L., Bastian, A., Bruder, D., & Reichl, U.Use of magnetic sulfated cellulose particles allows fast purification and blending of potent influenza vaccines in micePresented at: Recovery of Biological Products Conference SeriesBermuda, June 19, 2016.

Fortuna, A. R., Pieler, M., Hundt, B., Villain, L., Wolff, M. W., & Reichl, U.Membrane supports for influenza virus purificationPresented at: ISPPP 2014 - 34th International Symposium on the Separation of Proteins, Peptides and PolynucleotidesFortress Marienberg, Würzburg, Germany, November 5, 2014.

Fortuna, A. R., Pieler, M., Hundt, B., Villain, L., Wolff, M. W., & Reichl, U.Membrane supports for influenza virus purificationPresented at: ProcessNet-Jahrestagung und 31. DECHEMA-Jahrestagung der Biotechnologen 2014Aachen, Germany, September 30, 2014.

Pieler, M., Fortuna, A. R., Hundt, B., Reichl, U., & Wolff, M. W.Investigation of virus aggregation on performance of downstream processing in influenza vaccine manufacturingPresented at: ISPPP 2014 - 34th International Symposium on the Separation of Proteins, Peptides and PolynucleotidesFortress Marienberg, Würzburg, Germany, November 5, 2014.