Publications BPE group

Journal Article

Göbel, S.; Mayerlen, L.; Eiser, I. Y.; Fichtmüller, L.; Clements, D.; Reichl, U.; Genzel, Y.; Lehrer, A. T.: Process Intensification for Recombinant Marburg Virus Glycoprotein Production UsingDrosophilaS2 Cells. Engineering in Life Sciences 25 (5), e70022 (2025)

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Journal Article

Heyer, R.; Wolf, M.; Benndorf, D.; Uzzau, S.; Seifert, J.; Grenga, L.; Pabst, M.; Schmitt, H.; Mesuere, B.; Van Den Bossche, T. et al.; Haange, S.-B.; Jehmlich, N.; Di Luca, M.; Ferrer, M.; Serrano-Villar, S.; Armengaud, J.; Bode, H. B.; Hellwig, P.; Masselot, C. R.; Léonard, R.; Wilmes, P.: Metaproteomics in the One Health framework for unraveling microbial effectors in microbiomes. Microbiome 13, 134 (2025)

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Journal Article

Küchler, J.; Opitz, P.; Jordan, I.; Genzel, Y.; Benndorf, D.; Reichl, U.: Quantification of intracellular influenza A virus protein dynamics in different host cells after seed virus adaptation. Applied Microbiology and Biotechnology 109, 74 (2025)

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Journal Article

Matsuda, T.; Hoang, N.-H.: Medium engineering of lipase‐catalyzed reaction usingCO2. Journal of the American Oil Chemists' Society 102 (2), pp. 401 - 412 (2025)

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Journal Article

Mosqueda-Frómeta, O.; Andujar, I.; Mosqueda-Rodríguez, G. M.; Pestryakov, A.; Companioni, B.; Tapia y Figueroa, M. d. L.; Concepción, O.; Escalona, M.; Bogdanchikova, N.; Lorenzo, J. C.: Mini-review: Application of nanoparticles in plant biotechnology and agriculture. In Vitro Cellular & Developmental Biology - Plant (2025)

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Journal Article

Van Den Bossche, T.; Armengaud, J.; Benndorf, D.; Blakeley‐Ruiz, J. A.; Brauer, M.; Cheng, K.; Creskey, M.; Figeys, D.; Grenga, L.; Griffin, T. J. et al.; Henry, C.; Hettich, R. L.; Holstein, T.; Jagtap, P. D.; Jehmlich, N.; Kim, J.; Kleiner, M.; Kunath, B. J.; Malliet, X.; Martens, L.; Mehta, S.; Mesuere, B.; Ning, Z.; Tanca, A.; Uzzau, S.; Verschaffelt, P.; Wang, J.; Wilmes, P.; Zhang, X.; Zhang, X.; Li, L.: The microbiologist's guide to metaproteomics. iMeta 4 (3), e70031 (2025)

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Journal Article

Wolf, M.; Lange, J.; Benndorf, D.; Welz, L.; Nikolaus, S.; Siever, L. K.; Tran, F.; Schallert, K.; Hellwig, P.; Schreiber, S. et al.; Gunzer, M.; Rosenstiel, P.; Reichl, U.; Adolph, T.; Jukic, A.; Aden, K.; Heyer, R.: Fecal metaproteomics enables functional characterization of remission in patients with inflammatory bowel disease. Journal of Proteomics 318, 105455 (2025)

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Journal Article

Zinnecker, T. M.; Thiele, K.; Schmidberger, T.; Genzel, Y.; Reichl, U.: Influenza A Virus Production Following Quality by Design Principles. Engineering in Life Sciences 25 (4), e70027 (2025)

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Journal Article

Zinnecker, T. M.; Wicke, E.; Reichl, U.; Göbel, S.; Genzel, Y.: Seed Train Intensification and TFDF-Based Perfusion for MDCK Cell-Based Influenza A Virus Production. Processes 13 (5), 1286 (2025)

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Journal Article

Zuniga-Banuelos, F. J.; Hoffmann, M.; Reichl, U.; Rapp, E.: New Avenues for Human Blood Plasma Biomarker Discovery via Improved In-Depth Analysis of the Low-Abundant N-glycoproteome. Engineering (2025)

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2024

Journal Article

Arigoni-Affolter, I.; Losfeld, M.-E.; Hennig, R.; Rapp, E.; Aebi, M.: A hierarchical structure in the N-glycosylation process governs the N-glycosylation output: prolonged cultivation induces glycoenzymes expression variations that are reflected in the cellular N-glycome but not in the protein and site-specific glycoprofile of CHO cells. Glycobiology 34 (8), cwae045 (2024)

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Journal Article

Böttinger, K.; Regl, C.; Schäpertöns, V.; Rapp, E.; Wohlschlager, T.; Huber, C. G.: “Small is beautiful” – examining reliable determination of low- abundant therapeutic antibody glycovariants. Journal of Pharmaceutical Analysis 14 (10), 100982 (2024)

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Journal Article

Göbel, S.; Kazemi, O.; Ma, J.; Jordan, I.; Sandig, V.; Paulissen, J.; Kerstens, W.; Thibaut, H. J.; Reichl, U.; Dallmeier, K. et al.; Genzel, Y.: Parallel Multifactorial Process Optimization and Intensification for High-Yield Production of Live YF17D-Vectored Zika Vaccine. Vaccines 12 (7), 755 (2024)

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Journal Article

Göbel, S.; Pelz, L.; Silva, C. A. T.; Brühlmann, B.; Hill, C.; Altomonte, J.; Kamen, A.; Reichl, U.; Genzel, Y.: Production of recombinant vesicular stomatitis virus-based vectors by tangential flow depth filtration. Applied Microbiology and Biotechnology 108 (1), 240 (2024)

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Journal Article

Guzman Ruiz, L.; Zollner, A. M.; Hoxie, I.; Küchler, J.; Hausjell, C.; Mesurado, T.; Krammer, F.; Jungbauer, A.; Pereira Aguilar, P.; Klausberger, M. et al.; Grabherr, R.: Enhancing NA immunogenicity through novel VLP designs. Vaccine 42 (24), 126270 (2024)

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Journal Article

Hellwig, P.; Dittrich, A.; Heyer, R.; Reichl, U.; Benndorf, D.: Detection, isolation and characterization of phage-host complexes using BONCAT and click chemistry. Frontiers in Microbiology 15, 1434301 (2024)

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Journal Article

Hellwig, P.; Kautzner, D.; Heyer, R.; Dittrich, A.; Wibberg, D.; Busche, T.; Winkler, A.; Reichl, U.; Benndorf, D.: Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesized proteins. ISME Communications 4 (1), ycae153 (2024)

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Journal Article

Heyer, R.; Hellwig, P.; Maus, I.; Walke, D.; Schlüter, A.; Hassa, J.; Sczyrba, A.; Tubbesing, T.; Klocke, M.; Mächtig, T. et al.; Schallert, K.; Seick, I.; Reichl, U.; Benndorf, D.: Breakdown of hardly degradable carbohydrates (lignocellulose) in a two-stage anaerobic digestion plant is favored in the main fermenter. Water Research 250, 121020 (2024)

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Journal Article

Huber, N.; Alcalá Orozco, E. A.; Rexer, T.; Reichl, U.; Klamt, S.: Model-based optimization of cell-free enzyme cascades exemplified for the production of GDP-fucose. Metabolic Engineering 81, pp. 10 - 25 (2024)

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Journal Article

Karsten, C. B.; Buettner, F. F. R.; Cajic, S.; Nehlmeier, I.; Roshani, B.; Klippert, A.; Sauermann, U.; Stolte-Leeb, N.; Reichl, U.; Gerardy-Schahn, R. et al.; Rapp, E.; Stahl-Hennig, C.; Pöhlmann, S.: Macrophage- and CD4+ T cell-derived SIV differ in glycosylation, infectivity and neutralization sensitivity. PLOS Pathogens 20 (5), e1012190 (2024)

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Journal Article

Khesali Aghtaei, H.; Heyer, R.; Reichl, U.; Benndorf, D.: Improved biological methanation using tubular foam-bed reactor. Biotechnology for Biofuels and Bioproducts 17, 66 (2024)

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Journal Article

Offersgaard, A.; Duarte Hernandez, C. R.; Zhou, Y.; Duan, Z.; Gammeltoft, K. A.; Hartmann, K. T.; Fahnøe, U.; Marichal-Gallardo, P.; Alzua, G. P.; Underwood, A. P. et al.; Sølund, C.; Weis, N.; Bonde, J. H.; Christensen, J. P.; Pedersen, G. K.; Jensen, H. E.; Holmbeck, K.; Bukh, J.; Gottwein, J. M.: An inactivated SARS-CoV-2 vaccine based on a Vero cell culture-adapted high-titer virus confers cross-protection in small animals. Scientific Reports 14 (1), 17039 (2024)

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Journal Article

Pelz, L.; Dogra, T.; Marichal-Gallardo, P.; Hein, M. D.; Hemissi, G.; Kupke, S. Y.; Genzel, Y.; Reichl, U.: Production of antiviral “OP7 chimera” defective interfering particles free of infectious virus. Applied Microbiology and Biotechnology 108, 97 (2024)

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Journal Article

Riedel, R.; Commichau, F. M.; Benndorf, D.; Hertel, R.; Holzer, K.; Hoelzle, L. E.; Mardoukhi, M. S. Y.; Noack, L. E.; Martienssen, M.: Biodegradation of selected aminophosphonates by the bacterial isolate Ochrobactrum sp. BTU1. Microbiological Research 280, 127600 (2024)

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Journal Article

Riedel, R.; Meißner, K.; Kaschubowski, A.; Benndorf, D.; Martienssen, M.; Braun, B.: Laundry Isolate Delftia sp. UBM14 Capable of Biodegrading Industrially Relevant Aminophosphonates. Microorganisms 12 (8), 1664 (2024)

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Journal Article

Rüdiger, D.; Piasecka, J.; Küchler, J.; Pontes, C.; Laske, T.; Kupke, S. Y.; Reichl, U.: Mathematical model calibrated to in vitro data predicts mechanisms of antiviral action of the influenza defective interfering particle “OP7”. iScience 27 (4), 109421 (2024)

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Journal Article

Zinnecker, T. M.; Badri, N.; Araujo, D.; Thiele, K.; Reichl, U.; Genzel, Y.: From single‐cell cloning to high‐yield influenza virus production – implementing advanced technologies in vaccine process development. Engineering in Life Sciences 24 (4), 2300245 (2024)

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Journal Article

Zinnecker, T. M.; Reichl, U.; Genzel, Y.: Innovations in cell culture-based influenza vaccine manufacturing – from static cultures to high cell density cultivations. Human Vaccines & Immunotherapeutics 20 (1), 2373521 (2024)

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2023

Journal Article

Alcalá Orozco, E. A.; Grote, V.; Fiebig, T.; Klamt, S.; Reichl, U.; Rexer, T.: A Cell‐Free Multi‐enzyme Cascade Reaction for the Synthesis of CDP‐Glycerol. ChemBioChem: A European Journal of Chemical Biology 24 (21), e202300463 (2023)

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Journal Article

Burock, R.; Cajic, S.; Hennig, R.; Buettner, F. F. R.; Reichl, U.; Rapp, E.: Reliable N-Glycan Analysis–Removal of Frequently Occurring Oligosaccharide Impurities by Enzymatic Degradation. Molecules 28 (4), 1843 (2023)

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Journal Article

Cajic, S.; Hennig, R.; Grote, V.; Reichl, U.; Rapp, E.: Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches. Engineering 26, pp. 132 - 150 (2023)

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Journal Article

Dogra, T.; Pelz, L.; Boehme, J. D.; Küchler, J.; Kershaw, O.; Marichal-Gallardo, P.; Baelkner, M.; Hein, M. D.; Gruber, A. D.; Benndorf, D. et al.; Genzel, Y.; Bruder, D.; Kupke, S. Y.; Reichl, U.: Generation of “OP7 chimera” defective interfering influenza A particle preparations free of infectious virus that show antiviral efficacy in mice. Scientific Reports 13, 20936 (2023)

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Journal Article

Göbel, S.; Jaén, K. E.; Dorn, M.; Neumeyer, V.; Jordan, I.; Sandig, V.; Reichl, U.; Altomonte, J.; Genzel, Y.: Process intensification strategies toward cell culture‐based high‐yield production of a fusogenic oncolytic virus. Biotechnology and Bioengineering 120 (9), pp. 2639 - 2657 (2023)

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Journal Article

Göbel, S.; Jaén, K. E.; Fernandes, R. P.; Reiter, M.; Altomonte, J.; Reichl, U.; Genzel, Y.: Characterization of a quail suspension cell line for production of a fusogenic oncolytic virus. Biotechnology and Bioengineering 120, pp. 3335 - 3346 (2023)

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Journal Article

Hassa, J.; Tubbesing, T. J.; Maus, I.; Heyer, R.; Benndorf, D.; Effenberger, M.; Henke, C.; Osterholz, B.; Beckstette, M.; Pühler, A. et al.; Sczyrba, A.; Schlüter, A.: Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics. Microorganisms 11 (10), 2412 (2023)

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Journal Article

Hein, M. D.; Kazenmaier, D.; van Heuvel, Y.; Dogra, T.; Cattaneo, M.; Kupke, S. Y.; Stitz, J.; Genzel, Y.; Reichl, U.: Production of retroviral vectors in continuous high cell density culture. Applied Microbiology and Biotechnology 107, pp. 5947 - 5961 (2023)

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Journal Article

Hussnaetter, K. P.; Palm, P.; Pich, A.; Franzreb, M.; Rapp, E.; Elling, L.: Strategies for Automated Enzymatic Glycan Synthesis (AEGS). Biotechnology Advances 67, 108208 (2023)

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Journal Article

Küchler, J.; Willenbücher, K.; Reiß, E.; Nuß, L.; Conrady, M.; Ramm, P.; Schimpf, U.; Reichl, U.; Szewzyk, U.; Benndorf, D.: Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry. Fermentation 9 (1), 67 (2023)

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Journal Article

Morticelli, L.; Rossdam, C.; Cajic, S.; Böthig, D.; Magdei, M.; Tuladhar, S. R.; Petersen, B.; Fischer, K.; Rapp, E.; Korossis, S. et al.; Haverich, A.; Schnieke, A.; Niemann, H.; Buettner, F. F. R.; Hilfiker, A.: Genetic knockout of porcineGGTA1orCMAH/GGTA1is associated with the emergence of neo‐glycans. Xenotransplantation 30 (4), e12804 (2023)

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Journal Article

Offersgaard, A.; Duarte Hernandez, C. R.; Feng, S.; Marichal-Gallardo, P.; Holmbeck, K.; Pihl, A. F.; Fernandez-Antunez, C.; Peña Alzua, G.; Top Hartmann, K.; Pham, L. V. et al.; Zhou, Y.; Anbro Gammeltoft, K.; Fahnøe, U.; Schneider, U. V.; Pedersen, G. K.; Jensen, H. E.; Pravsgaard Christensen, J.; Ramirez, S.; Bukh, J.; Gottwein, J. M.: An inactivated SARS-CoV-2 vaccine induced cross-neutralizing persisting antibodies and protected against challenge in small animals. iScience 26 (2), 105949 (2023)

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Journal Article

Pelz, L.; Piagnani, E.; Marsall, P.; Wynserski, N.; Hein, M. D.; Marichal-Gallardo, P.; Kupke, S. Y.; Reichl, U.: Broad-Spectrum Antiviral Activity of Influenza A Defective Interfering Particles against Respiratory Syncytial, Yellow Fever, and Zika Virus Replication In Vitro. Viruses 15 (9), 1872 (2023)

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Journal Article

Trbojević-Akmačić, I.; Vučković, F.; Pribić, T.; Vilaj, M.; Černigoj, U.; Vidič, J.; Šimunović, J.; Kępka, A.; Kolčić, I.; Klarić, L. et al.; Novokmet, M.; Pučić-Baković, M.; Rapp, E.; Štrancar, A.; Polašek, O.; Wilson, J. F.; Lauc, G.: Comparative analysis of transferrin and IgG N-glycosylation in two human populations. Communications Biology 6, 312 (2023)

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Journal Article

van Heuvel, Y.; Schatz, S.; Hein, M.; Dogra, T.; Kazenmaier, D.; Tschorn, N.; Genzel, Y.; Stitz, J.: Novel suspension retroviral packaging cells generated by transposition using transposase encoding mRNA advance vector yields and enable production in bioreactors. Frontiers in Bioengineering and Biotechnology 11, 1076524 (2023)

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Journal Article

Wenzel, L.; Hoffmann, M.; Rapp, E.; Rexer, T.; Reichl, U.: Cell-free N-glycosylation of peptides using synthetic lipid-linked hybrid and complex N-glycans. Frontiers in Molecular Biosciences 10, 1266431 (2023)

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2022

Journal Article

Beimdiek, J.; Hennig, R.; Burock, R.; Puk, O.; Biskup, S.; Rapp, E.; Lesinski-Schiedat, A.; Buettner, F. F. R.; Das, A. M.: Serum N-glycomics of a novel CDG-IIb patient reveals aberrant IgG glycosylation. Glycobiology 32 (5), pp. 380 - 390 (2022)

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Journal Article

Göbel, S.; Kortum, F.; Chavez, K. J.; Jordan, I.; Sandig, V.; Reichl, U.; Altomonte, J.; Genzel, Y.: Cell-line screening and process development for a fusogenic oncolytic virus in small-scale suspension cultures. Applied Microbiology and Biotechnology 106, pp. 4945 - 4961 (2022)

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Journal Article

Hinterholzer, A.; Moises, J.; Regl, C.; Schwap, S.; Rapp, E.; Huber, C. G.; Schubert, M.: Unambiguous identification of α-Gal epitopes in intact monoclonal antibodies by NMR spectroscopy. mAbs 14 (1), 2132977 (2022)

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Journal Article

Hütte, H. J.; Tiemann, B.; Shcherbakova, A.; Grote, V.; Hoffmann, M.; Povolo, L.; Lommel, M.; Strahl, S.; Vakhrushev, S. Y.; Rapp, E. et al.; Buettner, F. F. R.; Halim, A.; Imberty, A.; Bakker, H.: A Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C- and O-Mannosylated Peptides. Analytical Chemistry 94 (20), pp. 7329 - 7338 (2022)

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Journal Article

Khesali Aghtaei, H.; Püttker, S.; Maus, I.; Heyer, R.; Huang, L.; Sczyrba, A.; Reichl, U.; Benndorf, D.: Adaptation of a microbial community to demand-oriented biological methanation. Biotechnology for Biofuels and Bioproducts 15, 125 (2022)

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