Publications of Robert Janke

Pech, S., Rehberg, M., Janke, R., Benndorf, D., Genzel, Y., Muth, T., Sickmann, A., Rapp, E., & Reichl, U. (2021)Tracking changes in adaptation to suspension growth for MDCK cells: cell growth correlates with levels of metabolites, enzymes and proteinsApplied Microbiology and Biotechnology, 105 (5), 1861–1874doi: 10.1007/s00253-021-11150-z.

Rath, A., Rehberg, M., Janke, R., Genzel, Y., Scholz, S., Noll, T., Rose, T., Sandig, V., & Reichl, U. (2014)The influence of cell growth and enzyme activity changes on intracellular metabolite dynamics in AGE1.HN.AAT cellsJournal of Biotechnology, 178, 43–53doi: 10.1016/j.jbiotec.2014.03.012.

Janke, R., Genzel, Y., Händel, N., Wahl, A., & Reichl, U. (2011)Metabolic adaptation of MDCK cells to different growth conditions: Effects on catalytic activities of central metabolic enzymesBiotechnology and Bioengineering, 108 (11), 2691–2704doi: 10.1002/bit.23215.

Janke, R., Genzel, Y., Freund, S., Wolff, M. W., Grammel, H., Rühmkorf, C., Seidemann, J., Wahl, A., & Reichl, U. (2010)Expression, purification, and characterization of a His6-tagged glycerokinase from Pichia farinosa for enzymatic cycling assays in mammalian cellsJournal of Biotechnology, 150 (3), 396–403doi: 10.1016/j.jbiotec.2010.09.963.

Janke, R., Genzel, Y., Wahl, A., & Reichl, U. (2010)Measurement of Key Metabolic Enzyme Activities in Mammalian Cells Using Rapid and Sensitive Microplate-Based AssaysBiotechnology and Bioengineering, 107 (3), 566–581doi: 10.1002/bit.22817.

Janke, R., Genzel, Y., Wetzel, M., & Reichl, U. (2011)Effect of influenza virus infection on key metabolic enzyme activities in MDCK cellsIn: BMC Proceedings, P129doi: 10.1186/1753-6561-5-S8-P129.

Janke, R., Genzel, Y., & Reichl, U.A high-throughput assay to assess enzyme activity in central metabolism of production cell linesPresented at: Cell Culture Engineering XIIIScottsdale, Arizona, USA, April 22, 2012.

Rath, A., Scholz, S., Noll, T., Janke, R., Genzel, Y., Rose, T., Sandig, V., & Reichl, U.Insights into mammalian energy metabolism: connecting intracellular metabolites and maximum enzyme activitiesPresented at: GVC/DECHEMA Vortrags- und Diskussionstagung - Biopharmazeutische ProduktionFreiburg, May 14, 2012.

Genzel, Y., Janke, R., Isken, B., Rödig, J., Rapp, E., Lohr, V., Behrendt, I., Scharfenberg, K., Kamen, A., Schiedner, G., Jordan, I., & Reichl, U.Influenza virus production: characteristics of different producer cell linesPresented at: 10th PEACe 2011Cascais, Portugal, September 25, 2011.

Janke, R., Genzel, Y., Wahl, A., & Reichl, U.Recombinant glycerokinase from Pichia farinosa: Intracellular expression, purification, characterization, and application.Presented at: 1st European Congress of Applied BiotechnologyBerlin, Germany, September 25, 2011.

Janke, R., Genzel, Y., Wahl, A., & Reichl, U.Influence of cell culture medium on the catalytic activity of key enzymes in MDCK cellsPresented at: 27. Jahrestagung der BiotechnologenMannheim, Germany, September 8, 2009.

Janke, R., Genzel, Y., Wahl, A., & Reichl, U.Effects of different growth conditions on the catalytic activities of central metabolic enzymes in MDCK cellsPresented at: 22nd ESACT MeetingVienna, Austria, May 15, 2011.

Rath, A., Prehm, C., Janke, R., Genzel, Y., Sandig, V., Rose, T., & Reichl, U.Intracellular metabolite pools and enzyme activities of AGE1.HN.AAT cells during continuous cultivationsPresented at: 22nd ESACT MeetingVienna, Austria, May 15, 2011.

Streif, S., Findeisen, R., Zeiger, L., Grammel, H., Janke, R., Genzel, Y., & Reichl, U.WPT7: Model Validation, Parameter Analysis and Experimental Design. Part I: Parameter estimation and model invalidation using uncertain data. Part II: Redox Signaling in the facultative photosynthetic bacterium Rhodospirillum rubrum. Part III: Sensitive cycling assays for measuring key metabolic enyzme activities in MDCK cells.Presented at: FORSYS/FORSYS Partner Status SeminarHeidelberg, Germany, February 15, 2011.

Janke, R., Genzel, Y., Wahl, A., & Reichl, U.Changes in specific enzyme activities of MDCK cells during growth under different cultivation conditionsPresented at: 8th European Symposium on Biochemical Engineering Science (ESBES)Bologna, Italy, September 6, 2010.

Janke, R., Genzel, Y., Wahl, A., & Reichl, U.Catalytic activity changes of key enzymes in MDCK cells under different growth conditionsPresented at: 21st ESACT MeetingDublin, Ireland, June 7, 2009.

Janke, R., Genzel, Y., Wahl, A., & Reichl, U.A high-throughput platform to measure key enzyme activities in mammalian cellsPresented at: German Symposium on Systems BiologyHeidelberg, Germany, May 12, 2009.

Kremling, A., Janke, R., Genzel, Y., Wahl, A., & Reichl, U.WPT7: Model Validation, Parameter Analysis and Experimental Design. Part I: Parameter estimation and parameter confidence intervalls. Part II: Key enzyme activities in central carbon metabolism of mammalian cells.Presented at: Evaluation and Status Seminar FORSYS 2009Heidelberg, Germany, October 6, 2009.

Janke, R., Wahl, A., & Reichl, U.Enzyme Activities in Central Carbon Metabolism of MDCK Cells under Different Growth ConditionsPresented at: BioPerspectivesHannover, Germany, October 7, 2008.

Janke, R., Wahl, A., & Reichl, U.Determination of key enzyme activities in the central carbon metabolism of MDCK cellsPresented at: ForSys MeetingBerlin, Germany, June 19, 2008.