Method Development for Multiplexing CGE-LIF Based Glycoanalysis
Proteomics Along the Adaptation of an Adherent MDCK Cell Line to Growth in Suspension
In cooperation with Prof. Scharfenberg at the Applied University Emden/Leer in Emden/Germany an adherent Madin-Darby canine kidney (MDCK) cell was adapted to growth in suspension. In animal cell culture growth in suspension and in chemically defined medium is desirable for many production processes. However, for vaccine production processes, a switch to growth in suspension is often challenging, as many viruses seem to depend on cell lines difficult to adapt. Furthermore, cell-specific productivity is often reduced after adaptation.
In this project, adherent growth versus growth in suspension for a MDCK cell line was investigated on the proteome level. Interestingly, significant changes on the protein level during adaptation of an adherent MDCK cell to growth in suspension could be identified. Differences in proteomic profile were detected during cell line adaptation as well as during exponential and stationary growth of the adherent and the suspension cell line. Most of the differentially expressed proteins were subsequently identified. The findings gave new insights into key characteristics of cell line adaptation to growth in suspension.