Publications

2.

Journal Article

Hellinga, A. H.; Cajic, S.; Linde, H. F.; van Stigt, A. H.; de Vries, J. H. M.; Brouwer‐Brolsma, E. M.; Hennig, R.; Rapp, E.; Mank, M.; Stahl, B. et al.; Kraneveld, A. D.; Leusen, J. H. W.; Bont, L.; van't Land, B.: Maternal Dietary Fiber Intake During Lactation and Human Milk Oligosaccharide Fucosylation: a PRIMA Birth Cohort Study. Molecular Nutrition & Food Research 69 (20), e70165 (2025)

MPG.PuRe

3.

Journal Article

Zuniga-Banuelos, F. J.; Hoffmann, M.; Reichl, U.; Rapp, E.: New Avenues for Human Blood Plasma Biomarker Discovery via Improved In-Depth Analysis of the Low-Abundant N-glycoproteome. Engineering (2025)

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4.

Journal Article

Zuniga-Banuelos, F. J.; Lemke, G.; Hoffmann, M.; Reichl, U.; Rapp, E.: Immunoglobulin A Carries Sulfated and O-acetylated N-glycans Primarily at the Tailpiece Site -Strategies for Site-Specific N-glycan Identification. Frontiers in Molecular Biosciences 12, 1595173 (2025)

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External

5.

Journal Article

Arigoni-Affolter, I.; Losfeld, M.-E.; Hennig, R.; Rapp, E.; Aebi, M.: A hierarchical structure in the N-glycosylation process governs the N-glycosylation output: prolonged cultivation induces glycoenzymes expression variations that are reflected in the cellular N-glycome but not in the protein and site-specific glycoprofile of CHO cells. Glycobiology 34 (8), cwae045 (2024)

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6.

Journal Article

Böttinger, K.; Regl, C.; Schäpertöns, V.; Rapp, E.; Wohlschlager, T.; Huber, C. G.: “Small is beautiful” – examining reliable determination of low- abundant therapeutic antibody glycovariants. Journal of Pharmaceutical Analysis 14 (10), 100982 (2024)

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7.

Journal Article

Karsten, C. B.; Buettner, F. F. R.; Cajic, S.; Nehlmeier, I.; Roshani, B.; Klippert, A.; Sauermann, U.; Stolte-Leeb, N.; Reichl, U.; Gerardy-Schahn, R. et al.; Rapp, E.; Stahl-Hennig, C.; Pöhlmann, S.: Macrophage- and CD4+ T cell-derived SIV differ in glycosylation, infectivity and neutralization sensitivity. PLOS Pathogens 20 (5), e1012190 (2024)

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8.

Journal Article

Burock, R.; Cajic, S.; Hennig, R.; Buettner, F. F. R.; Reichl, U.; Rapp, E.: Reliable N-Glycan Analysis–Removal of Frequently Occurring Oligosaccharide Impurities by Enzymatic Degradation. Molecules 28 (4), 1843 (2023)

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9.

Journal Article

Cajic, S.; Hennig, R.; Grote, V.; Reichl, U.; Rapp, E.: Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches. Engineering 26, pp. 132 - 150 (2023)

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10.

Journal Article

Hussnaetter, K. P.; Palm, P.; Pich, A.; Franzreb, M.; Rapp, E.; Elling, L.: Strategies for Automated Enzymatic Glycan Synthesis (AEGS). Biotechnology Advances 67, 108208 (2023)

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11.

Journal Article

Morticelli, L.; Rossdam, C.; Cajic, S.; Böthig, D.; Magdei, M.; Tuladhar, S. R.; Petersen, B.; Fischer, K.; Rapp, E.; Korossis, S. et al.; Haverich, A.; Schnieke, A.; Niemann, H.; Buettner, F. F. R.; Hilfiker, A.: Genetic knockout of porcineGGTA1orCMAH/GGTA1is associated with the emergence of neo‐glycans. Xenotransplantation 30 (4), e12804 (2023)

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12.

Journal Article

Trbojević-Akmačić, I.; Vučković, F.; Pribić, T.; Vilaj, M.; Černigoj, U.; Vidič, J.; Šimunović, J.; Kępka, A.; Kolčić, I.; Klarić, L. et al.; Novokmet, M.; Pučić-Baković, M.; Rapp, E.; Štrancar, A.; Polašek, O.; Wilson, J. F.; Lauc, G.: Comparative analysis of transferrin and IgG N-glycosylation in two human populations. Communications Biology 6, 312 (2023)

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13.

Journal Article

Wenzel, L.; Hoffmann, M.; Rapp, E.; Rexer, T.; Reichl, U.: Cell-free N-glycosylation of peptides using synthetic lipid-linked hybrid and complex N-glycans. Frontiers in Molecular Biosciences 10, 1266431 (2023)

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14.

Journal Article

Beimdiek, J.; Hennig, R.; Burock, R.; Puk, O.; Biskup, S.; Rapp, E.; Lesinski-Schiedat, A.; Buettner, F. F. R.; Das, A. M.: Serum N-glycomics of a novel CDG-IIb patient reveals aberrant IgG glycosylation. Glycobiology 32 (5), pp. 380 - 390 (2022)

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15.

Journal Article

Hinterholzer, A.; Moises, J.; Regl, C.; Schwap, S.; Rapp, E.; Huber, C. G.; Schubert, M.: Unambiguous identification of α-Gal epitopes in intact monoclonal antibodies by NMR spectroscopy. mAbs 14 (1), 2132977 (2022)

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16.

Journal Article

Hütte, H. J.; Tiemann, B.; Shcherbakova, A.; Grote, V.; Hoffmann, M.; Povolo, L.; Lommel, M.; Strahl, S.; Vakhrushev, S. Y.; Rapp, E. et al.; Buettner, F. F. R.; Halim, A.; Imberty, A.; Bakker, H.: A Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C- and O-Mannosylated Peptides. Analytical Chemistry 94 (20), pp. 7329 - 7338 (2022)

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17.

Journal Article

Lageveen-Kammeijer, G. S. M.; Rapp, E.; Chang, D.; Rudd, P. M.; Kettner, C.; Zaia, J.: The minimum information required for a glycomics experiment (MIRAGE): reporting guidelines for capillary electrophoresis. Glycobiology 32 (7), pp. 580 - 587 (2022)

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18.

Journal Article

Tolonen, A. C.; Beauchemin, N.; Bayne, C.; Li, L.; Tan, J.; Lee, J.; Meehan, B. M.; Meisner, J.; Millet, Y.; LeBlanc, G. et al.; Kottler, R.; Rapp, E.; Murphy, C.; Turnbaugh, P. J.; von Maltzahn, G.; Liu, C. M.; van Hylckama Vlieg, J. E. T.: Synthetic glycans control gut microbiome structure and mitigate colitis in mice. Nature Communications 13 (1), 1244 (2022)

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19.

Journal Article

Chuzel, L.; Fossa, S. L.; Boisvert, M. L.; Cajic, S.; Hennig, R.; Ganatra, M. B.; Reichl, U.; Rapp, E.; Taron, C. H.: Combining functional metagenomics and glycoanalytics to identify enzymes that facilitate structural characterization of sulfated N-glycans. Microbial Cell Factories 20 (20), 162 (2021)

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20.

Journal Article

Di Marco, F.; Berger, T.; Esser-Skala, W.; Rapp, E.; Regl, C.; Huber, C. G.: Simultaneous Monitoring of Monoclonal Antibody Variants by Strong Cation-Exchange Chromatography Hyphenated to Mass Spectrometry to Assess Quality Attributes of Rituximab-Based Biotherapeutics. International Journal of Molecular Sciences 22 (16), 9072 (2021)

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21.

Journal Article

Eussen, S. R. B. M.; Mank, M.; Kottler, R.; Hoffmann, X.-K.; Behne, A.; Rapp, E.; Stahl, B.; Mearin, M. L.; Koletzko, B.: Presence and Levels of Galactosyllactoses and Other Oligosaccharides in Human Milk and Their Variation during Lactation and According to Maternal Phenotype. Nutrients 13 (7), 2324 (2021)

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22.

Journal Article

Feichtinger, R. G.; Hüllen, A.; Koller, A.; Kotzot, D.; Grote, V.; Rapp, E.; Hofbauer, P.; Brugger, K.; Thiel, C.; Mayr, J. A. et al.; Wortmann, S. B.: A spoonful of L‐fucose—an efficient therapy for GFUS‐CDG, a new glycosylation disorder. Embo Molecular Medicine 13 (9), e14332 (2021)

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23.

Journal Article

Gücüm, S.; Sakson, R.; Hoffmann, M.; Grote, V.; Becker, C.; Pakari, K.; Beedgen, L.; Thiel, C.; Rapp, E.; Ruppert, T. et al.; Thumberger, T.; Wittbrodt, J.: A patient-based medaka alg2 mutant as a model for hypo-N-glycosylation. Development 148 (11), dev199385 (2021)

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24.

Journal Article

Kawahara, R.; Chernykh, A.; Alagesan, K.; Bern, M.; Cao, W.; Chalkley, R. J.; Cheng, K.; Choo, M. S.; Edwards, N.; Goldman, R. et al.; Hoffmann, M.; Hu, Y.; Huang, Y.; Kim, J. Y.; Kletter, D.; Liquet, B.; Liu, M.; Mechref, Y.; Meng, B.; Neelamegham, S.; Nguyen-Khuong, T.; Nilsson, J.; Pap, A.; Park, G. W.; Parker, B. L.; Pegg, C. L.; Penninger, J. M.; Phung, T. K.; Pioch, M.; Rapp, E.; Sakalli, E.; Sanda, M.; Schulz, B. L.; Scott, N. E.; Sofronov, G.; Stadlmann, J.; Vakhrushev, S. Y.; Woo, C. M.; Wu, H.-Y.; Yang, P.; Ying, W.; Zhang, H.; Zhang, Y.; Zhao, J.; Zaia, J.; Haslam, S. M.; Palmisano, G.; Yoo, J. S.; Larson, G.; Khoo, K.-H.; Medzihradszky, K. F.; Kolarich, D.; Packer, N. H.; Thaysen-Andersen, M.: Community evaluation of glycoproteomics informatics solutions reveals high-performance search strategies for serum glycopeptide analysis. Nature Methods 18, pp. 1304 - 1316 (2021)

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25.

Journal Article

Noor, S. I.; Hoffmann, M.; Rinis, N.; Bartels, M. F.; Winterhalter, P. R.; Hoelscher, C.; Hennig, R.; Himmelreich, N.; Thiel, C.; Ruppert, T. et al.; Rapp, E.; Strahl, S.: Glycosyltransferase POMGNT1 deficiency strengthens N-cadherin-mediated cell-cell adhesion. Journal of Biological Chemistry 296, 100433 (2021)

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26.

Journal Article

Patenaude, A.; Erhardt, J.; Hennig, R.; Rapp, E.; Lauc, G.; Pezer, M.: N‐glycosylation analysis of mouse immunoglobulin G isolated from dried blood spots. Electrophoresis: Special Issue: Monoliths 2021 42 (24), pp. 2615 - 2618 (2021)

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27.

Journal Article

Pech, S.; Rehberg, M.; Janke, R.; Benndorf, D.; Genzel, Y.; Muth, T.; Sickmann, A.; Rapp, E.; Reichl, U.: Tracking changes in adaptation to suspension growth for MDCK cells: cell growth correlates with levels of metabolites, enzymes and proteins. Applied Microbiology and Biotechnology 105 (5), pp. 1861 - 1874 (2021)

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28.

Journal Article

Pralow, A.; Hoffmann, M.; Nguyen-Khuong, T.; Pioch, M.; Hennig, R.; Genzel, Y.; Rapp, E.; Reichl, U.: Comprehensive N‐glycosylation analysis of the influenza A virus proteins HA and NA from adherent and suspension MDCK cells. The FEBS Journal 288 (16), pp. 4869 - 4891 (2021)

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29.

Journal Article

Pralow, A.; Nikolay, A.; Leon, A.; Genzel, Y.; Rapp, E.; Reichl, U.: Site-specific N-glycosylation analysis of animal cell culture-derived Zika virus proteins. Scientific Reports 11 (1), 5147 (2021)

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30.

Journal Article

Puan, K. J.; San Luis, B.; Yusof, N.; Kumar, D.; Andiappan, A. K.; Lee, W.; Cajic, S.; Vuckovic, D.; Chan, J. D.; Döllner, T. et al.; Hou, H. W.; Jiang, Y.; Tian, C.; Agee, M.; Aslibekyan, S.; Auton, A.; Babalola, E.; Bell, R. K.; Bielenberg, J.; Bryc, K.; Bullis, E.; Cameron, B.; Coker, D.; Partida, G. C.; Dhamija, D.; Das, S.; Elson, S. L.; Filshtein, T.; Fletez-Brant, K.; Fontanillas, P.; Freyman, W.; Gandhi, P. M.; Heilbron, K.; Hicks, B.; Hinds, D. A.; Huber, K. E.; Jewett, E. M.; Kleinman, A.; Kukar, K.; Lane, V.; Lin, K.-H.; Lowe, M.; Luff, M. K.; McCreight, J. C.; McIntyre, M. H.; McManus, K. F.; Micheletti, S. J.; Moreno, M. E.; Mountain, J. L.; Mozaffari, S. V.; Nandakumar, P.; Noblin, E. S.; O’Connell, J.; Petrakovitz, A. A.; Poznik, G. D.; Schumacher, M.; Shastri, A. J.; Shelton, J. F.; Shi, J.; Shringarpure, S.; Tran, V.; Tung, J. Y.; Wang, X.; Wang, W.; Weldon, C. H.; Wilton, P.; Rapp, E.; Poidinger, M.; Wang, D. Y.; Soranzo, N.; Lee, B.; Rötzschke, O.: FUT6 deficiency compromises basophil function by selectively abrogating their sialyl-Lewis x expression. Communications Biology 4, 832 (2021)

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31.

Journal Article

Ruhnau, J.; Grote, V.; Juárez-Osorio, M.; Bruder, D.; Mahour, R.; Rapp, E.; Rexer, T.; Reichl, U.: Cell-Free Glycoengineering of the Recombinant SARS-CoV-2 Spike Glycoprotein. Frontiers in Bioengineering and Biotechnology 9, 699026 (2021)

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32.

Journal Article

Hinneburg , H.; Pedersen, J. L.; Bokil, N. J.; Pralow, A.; Schirmeister, F.; Kawahara, R.; Rapp, E.; Saunders, B. M.; Thaysen-Andersen, M.: High-resolution Longitudinal N- And O-glycoprofiling of Human Monocyte-To- Macrophage Transition. Glycobiology 30 (9), pp. 679 - 694 (2020)

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33.

Journal Article

Rexer, T.; Wenzel, L.; Hoffmann, M.; Tischlik, S.; Bergmann, C.; Grote, V.; Boecker, S.; Bettenbrock, K.; Schildbach, A.; Kottler, R. et al.; Mahour, R.; Rapp, E.; Pietzsch, M.; Reichl, U.: Synthesis of lipid-linked oligosaccharides by a compartmentalized multi-enzyme cascade for the in vitro N-glycosylation of peptides. Journal of Biotechnology 322 (10), pp. 54 - 65 (2020)

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34.

Journal Article

Schaffert, A.; Hanić, M.; Novokmet, M.; Zaytseva, O.; Krištić, J.; Lux, A.; Nitschke, L.; Peipp, M.; Pezer, M.; Hennig, R. et al.; Rapp, E.; Lauc, G.; Nimmerjahn, F.: Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo. Frontiers in Immunology 10, 3024 (2020)

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35.

Journal Article

Bule, P.; Chuzel, L.; Blagova, E.; Wu, L.; Gray, M. A.; Henrissat, B.; Rapp, E.; Bertozzi, C. R.; Taron, C. H.; Davies, G. J.: Inverting family GH156 sialidases define an unusual catalytic motif for glycosidase action. Nature Communications 10, 4816 (2019)

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36.

Journal Article

Campbell, M.P.; Abrahams, J.L.; Rapp, E.; Struwe, W.B.; Costello, C.E.; Novotny, M.; Ranzinger, R.; York, W.S.; Kolarich, D.; Rudd, P.M. et al.; Kettner, C.: The minimum information required for a glycomics experiment (MIRAGE) project: LC guidelines. Glycobiology 29 (5), pp. 349 - 354 (2019)

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37.

Journal Article

Fischöder, T.; Cajic, S.; Grote, V.; Heinzler, R.; Reichl, U.; Franzreb, M.; Rapp, E.; Elling, L.: Enzymatic Cascades for Tailored 13C6 and 15N Enriched Human Milk Oligosaccharides. Molecules 24 (19), 3482 (2019)

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38.

Journal Article

Fischöder, T.; Cajic, S.; Reichl, U.; Rapp, E.; Elling , L.: Enzymatic cascade synthesis provides novel linear human milk oligosaccharides as reference standards for xCGE-LIF based high-throughput analysis. Biotechnology Journal 14 (3), 1800305 (2019)

MPG.PuRe

39.

Journal Article

Gränicher, G.; Coronel, J.; Pralow, A.; Marichal-Gallardo, P.; Wolff, M. W.; Rapp, E.; Karlas, A.; Sandig, V.; Genzel, Y.; Reichl, U.: Efficient influenza A virus production in high cell density using the novel porcine suspension cell line PBG.PK2.1. Vaccine 37 (47), pp. 7019 - 7028 (2019)

MPG.PuRe

40.

Journal Article

Hinneburg, H.; Chatterjee, S.; Schirmeister, F.; Nguyen-Khoung, T.; Packer, N.; Rapp, E.; Thaysen-Andersen, M.: Post-Column Make-Up Flow (PCMF) Enhances the Performance of Capillary-Flow PGC-LC-MS/MS-Based Glycomics. Analytical Chemistry 91 (7), pp. 4559 - 4567 (2019)

MPG.PuRe

41.

Journal Article

Munoz, R.I.; Kaehne, T.; Herrera, H.; Rodriguez, S.; Guerra, M.M.; Vio , K.; Hennig, R.; Rapp, E.; Rodriguez, E.: The subcommissural organ and the Reissner fiber: old friends revisited. Cell and Tissue Research 375 (2), pp. 507 - 529 (2019)

MPG.PuRe

42.

Journal Article

Reiding, K.R.; Bondt, A.; Hennig, R.; Gardner, R.A.; O'Flaherty, R.; Trbojević-Akmačić, I.; Shubhakar, A.; Hazes, J.; Reichl, U.; Fernandes, D.L. et al.; Pucic-Bakovic, M.; Rapp, E.; Spencer, D.I.R.; Dolhain, R.; Rudd, P.; Lauc, G.; Wuhrer, M.: High-throughput serum N-glycomics: method comparison and application to study rheumatoid arthritis and pregnancy-associated changes. Molecular and Cellular Proteomics 18 (1), pp. 3 - 15 (2019)

MPG.PuRe

43.

Journal Article

Rossdam, C.; Konze, S.; Oberbeck, A.; Rapp, E.; Gerardy-Schahn, R.; von Itzstein, M.; Buettner, F.: Approach for Profiling of Glycosphingolipid Glycosylation by Multiplexed Capillary Gel Electrophoresis Coupled to Laser-Induced Fluorescence Detection to Identify Cell-Surface Markers of Human Pluripotent Stem Cells and Derived Cardiomyocytes. Analytical Chemistry 91 (10), pp. 6413 - 6418 (2019)

MPG.PuRe

44.

Journal Article

Chuzel, L.; Ganatra, M. B.; Rapp, E.; Henrissat, B.; Taron, C. H.: Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156). The Journal of Biological Chemistry 293, pp. 18138 - 18150 (2018)

MPG.PuRe

45.

Journal Article

Clerc, F.; Novokmet, M.; Dotz, V.; Reiding, K. R.; de Haan , N.; Kammeijer, G. S.M.; Dalebout, H.; Bladergroen, M. R.; Vukovic, F.; Rapp, E. et al.; Targan, S. R.; Barron, G.; Manetti , N.; Latiano, A.; McGovern, D. P.B.; Annese, V.; Lauc, G.; Wuhrer, M.: Plasma N-Glycan Signatures Are Associated With Features of Inflammatory Bowel Diseases. Gastroenterology 155 (3), pp. 829 - 843 (2018)

MPG.PuRe

46.

Journal Article

Hoffmann, M.; Pioch, M.; Pralow, A.; Hennig, R.; Kottler, R.; Reichl, U.; Rapp, E.: The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses – Exploiting the Diagnostic Potential of Fragment Ions. Proteomics 18 (24), e1800282 (2018)

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supplementary-material

47.

Journal Article

Mahour, R.; Klapproth, J.; Rexer, T.; Schildbach, A.; Klamt, S.; Pietzsch, M.; Rapp, E.; Reichl, U.: Establishment of a five-enzyme cell-free cascade for the synthesis of uridine diphosphate N-acetylglucosamine. Journal of Biotechnology 283, pp. 120 - 129 (2018)

MPG.PuRe

48.

Journal Article

Möginger, U.; Grunewald, S.; Hennig, R.; Kuo, C.-W.; Schirmeister, F.; Voth, H.; Rapp, E.; Khoo, K.-H.; Seeberger, P. H.; Simon, J. C. et al.; Kolarich, D.: Alterations of the Human Skin N- and O-Glycome in Basal Cell Carcinoma and Squamous Cell Carcinoma. Frontiers in Oncology 8, 70 (2018)

MPG.PuRe

49.

Journal Article

Muth, T.; Kohrs, F.; Heyer, R.; Benndorf, D.; Rapp, E.; Reichl, U.; Martens, L.; Renard, B. Y.: MPA Portable: A Stand-Alone Software Package for Analyzing Metaproteome Samples on the Go. Analytical Chemistry 90, pp. 685 - 689 (2018)

MPG.PuRe

50.

Journal Article

Nguyen-Khuong, T.; Pralow, A.; Reichl, U.; Rapp, E.: Improvement of electrospray stability in negative ion mode for nano-PGC-LC-MS glycoanalysis via post-column make-up flow. Glycoconjugate Journal 35 (6), pp. 499 - 509 (2018)

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