Design and Optimization of Cell-free Production Systems
Computer-aided analysis, design and optimization of cell-free production systems has become a new research field for the ARB group. Cell-free bioproduction systems represent a promising alternative to classical microbial fermentation processes for synthesizing value-added chemicals. Important aspects of our research are:
- Identification of suitable metabolic modules with specified stoichiometric and thermodynamic constraints which can serve as cofactor regeneration modules for cell-free systems.
- Combining kinetic modeling and experimental investigations for model-based optimization of cell-free production systems.
- Application: production of nucleotide sugars (collaboration with BPE group at MPI Magdeburg).
- Application: enzyme cascades utilizing CO2 for synthesis of value-added products (with Biberach University for Applied Sciences).