Applications of Model-Driven Metabolic Engineering
We combine computational (dry-lab; see project area 1) and genetic (wet-lab) techniques to test and establish new metabolic engineering strategies and to construct microbial cell factories for the bio-based production of selected chemicals. As relevant host organism we focus on E. coli and, more recently, also on yeast (S. cerevisiae). Research topics include:
- Model-based metabolic engineering of E. coli for itaconic acid synthesis.
- Model-based metabolic engineering of E. coli for synthesis of succinate.
- Increasing the productivity of yeast (S. cerevisiae).
- Metabolic modeling of microbial communities involved in anaerobic digestion.
- Use of Zymomonas mobilis for synthesizing acetaldehyde.
- Metabolic engineering strategies for cyanobacteria.
- Design and analysis of cell-free enzymatic systems.
- New design principles for metabolic engineering (see StrainBooster project).
- Design of cell factories for two-stage processes based on dynamic metabolic control.