Max Planck Institute for Dynamics of Complex Technical Systems
Human Blood N-Glycoproteomics
The aim of this project is to detect disease-associated differences in the N-glycosylation of blood plasma glycoproteins derived from cohorts of clinical interest. It is intended that an in-depth N-glycoproteomic analysis will provide insights into the occupancy of N-glycosylation sites per protein (macro-heterogeneity), including the diversity and abundance of N-glycans bound to each N-glycosylation site (micro-heterogeneity). Through an integrative statistical analysis of N-glycoproteomic datasets it is anticipated to detect N-glycosylation-based biomarkers (Figure 1).
Hoffmann, M.; Marx, K.; Reichl, U.; Wuhrer, M.; Rapp, E.: Site-specific O-Glycosylation Analysis of Human Blood Plasma Proteins. Molecular and Cellular Proteomics 15 (2), pp. 624 - 641 (2016)
Pioch, M.; Hoffmann, M.; Pralow, A.; Reichl, U.; Rapp, E.: GlyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data. Analytical Chemistry 90 (20), pp. 11908 - 11916 (2018)
Hoffmann, M.; Pioch, M.; Pralow, A.; Hennig, R.; Kottler, R.; Reichl, U.; Rapp, E.: The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses – Exploiting the Diagnostic Potential of Fragment Ions. Proteomics 18 (24), e1800282 (2018)