Team Leader (BPA)

Dr. Erdmann Rapp
Dr. Erdmann Rapp
Phone: +49 391 6110 314
Fax: +49 391 6110 535
Room: S0.13

Researchers

Dipl.-Ing. Marcus Hoffmann
Phone: +49 391 6110 427
Room: S0.07

glyXera GmbH:

M. Sc. Frania Zuniga, Ph. D. Student
Phone: +49 391 6110 363

Alumni:

Markus Pioch, M. Sc. (FH)
Phone: +49 391 6110 399
Room: S0.09

Human Blood N-Glycoproteomics

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Human Blood N-Glycoproteomics

The aim of this project is to detect disease-associated differences in the N-glycosylation of blood plasma glycoproteins derived from cohorts of clinical interest. It is intended that an in-depth N-glycoproteomic analysis will provide insights into the occupancy of N-glycosylation sites per protein (macro-heterogeneity), including the diversity and abundance of N-glycans bound to each N-glycosylation site (micro-heterogeneity). Through an integrative statistical analysis of N-glycoproteomic datasets it is anticipated to detect N-glycosylation-based biomarkers (Figure 1).

Figure 1: N-Glycoproteomic workflow for the discovery of disease-associated N-glycosylation alterations in human blood plasma glycoproteins. Zoom Image
Figure 1: N-Glycoproteomic workflow for the discovery of disease-associated N-glycosylation alterations in human blood plasma glycoproteins. [less]

Publications

Hoffmann, M.; Marx, K.; Reichl, U.; Wuhrer, M.; Rapp, E.: Site-specific O-Glycosylation Analysis of Human Blood Plasma Proteins. Molecular and Cellular Proteomics 15 (2), pp. 624 - 641 (2016)
Pioch, M.; Hoffmann, M.; Pralow, A.; Reichl, U.; Rapp, E.: GlyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data. Analytical Chemistry 90 (20), pp. 11908 - 11916 (2018)
Hoffmann, M.; Pioch, M.; Pralow, A.; Hennig, R.; Kottler, R.; Reichl, U.; Rapp, E.: The Fine Art of Destruction: A Guide to In-Depth Glycoproteomic Analyses – Exploiting the Diagnostic Potential of Fragment Ions. Proteomics 18 (24), e1800282 (2018)
 
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