Analysis of Virus-Host Cell Interaction on the Proteome Level

Motivation

This project is related to other genomic and metabolic approaches, all focusing on the dynamic interaction of the virus with mammalian host cells during vaccine production. Therefore, changes in the expression of host cell proteins in various mammalian cells after infection with different virus strains are characterized. In addition, proteome changes related to cultivation conditions are investigated.

Figure A: Process scheme for differential protein abundance analysis and protein identification, using the 2D-DIGE system and nanoHPLC-MS/MS

© MPI Magdeburg (BPE)

**Figure A:** Process scheme for differential protein abundance analysis and protein identification, using the 2D-DIGE system and nanoHPLC-MS/MS

© MPI Magdeburg (BPE)

Aim of the project

With two dimensional differential gel electrophoresis and mass spectrometry (Figure A) we detect differentially expressed host cell proteins over the time course of an infection phase. Different cell lines, like Vero and MDCK, are hosts for cell-based vaccine production. The results are correlated with integrated process data and gave detailed insight into the vaccine production process on the cellular level. This knowledge contributes to a better characterization und understanding of the complex virus-host interaction. Regulated proteins could be target for genetic engineering of host cells to increase viral titers.

References

Vester, D.; Rapp, E.; Gade, D.; Genzel, Y.; Reichl, U.: Quantitative analysis of cellular proteome alterations in human influenza A virus-infected mammalian cell lines. Proteomics 9 (12), pp. 3316 - 3327 (2009)

MPG.PuRe

DOI

Vester, D.; Rapp, E.; Kluge, S.; Genzel, Y.; Reichl, U.: Virus-host cell interactions in vaccine production cell lines infected with different human influenza A virus variants: A proteomic approach. Journal of Proteomics 73 (9), pp. 1656 - 1669 (2010)

MPG.PuRe

DOI

Kluge, S.; Rourou, S.; Vester, D.; Majoul, S.; Benndorf, D.; Genzel, Y.; Rapp, E.; Kallel, H.; Reichl, U.: Proteome analysis of virus-host cell interaction: rabies virus replication in Vero cells in two different media. Applied Microbiology and Biotechnology 97 (12), pp. 5493 - 5506 (2013)

MPG.PuRe

DOI