Publications of Pavel Marichal-Gallardo

Journal Article (13)

2024
Journal Article
Pelz, L.; Dogra, T.; Marichal-Gallardo, P.; Hein, M. D.; Hemissi, G.; Kupke, S. Y.; Genzel, Y.; Reichl, U.: Production of antiviral “OP7 chimera” defective interfering particles free of infectious virus. Applied Microbiology and Biotechnology 108, pp. 1 - 15 (2024)
2023
Journal Article
Dogra, T.; Pelz, L.; Boehme, J. D.; Küchler, J.; Kershaw, O.; Marichal-Gallardo, P.; Baelkner, M.; Hein, M. D.; Gruber, A. D.; Benndorf, D. et al.; Genzel, Y.; Bruder, D.; Kupke, S. Y.; Reichl, U.: Generation of “OP7 chimera” defective interfering influenza A particle preparations free of infectious virus that show antiviral efficacy in mice. Scientific Reports 13, 20936 (2023)
Journal Article
Offersgaard, A.; Duarte Hernandez, C. R.; Feng, S.; Marichal-Gallardo, P.; Holmbeck, K.; Pihl, A. F.; Fernandez-Antunez, C.; Peña Alzua, G.; Top Hartmann, K.; Pham, L. V. et al.; Zhou, Y.; Anbro Gammeltoft, K.; Fahnøe, U.; Schneider, U. V.; Pedersen, G. K.; Jensen, H. E.; Pravsgaard Christensen, J.; Ramirez, S.; Bukh, J.; Gottwein, J. M.: An inactivated SARS-CoV-2 vaccine induced cross-neutralizing persisting antibodies and protected against challenge in small animals. iScience 26 (2), 105949 (2023)
Journal Article
Pelz, L.; Piagnani, E.; Marsall, P.; Wynserski, N.; Hein, M. D.; Marichal-Gallardo, P.; Kupke, S. Y.; Reichl, U.: Broad-Spectrum Antiviral Activity of Influenza A Defective Interfering Particles against Respiratory Syncytial, Yellow Fever, and Zika Virus Replication In Vitro. Viruses 15 (9), 1872 (2023)
2021
Journal Article
Bissinger, T.; Wu, Y.; Marichal-Gallardo, P.; Riedel, D.; Liu, X.; Genzel, Y.; Tan, W.; Reichl, U.: Towards integrated production of an influenza A vaccine candidate with MDCK suspension cells. Biotechnology and Bioengineering 118 (10), pp. 3996 - 4013 (2021)
Journal Article
Gränicher, G.; Babakhani, M.; Göbel, S.; Jordan, I.; Marichal-Gallardo, P.; Genzel, Y.; Reichl, U.: A high cell density perfusion process for Modified Vaccinia virus Ankara production: process integration with inline DNA digestion and cost analysis. Biotechnology and Bioengineering 118 (12), pp. 4720 - 4734 (2021)
Journal Article
Hein, M. D.; Arora, P.; Marichal-Gallardo, P.; Winkler, M.; Genzel, Y.; Pöhlmann, S.; Schughart, K.; Kupke, S. Y.; Reichl, U.: Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particles. BMC Biology 19 (1), 91 (2021)
Journal Article
Hein, M. D.; Kollmus, H.; Marichal-Gallardo, P.; Püttker, S.; Benndorf, D.; Genzel, Y.; Schughart, K.; Kupke, S. Y.; Reichl, U.: OP7, a novel influenza A virus defective interfering particle: production, purification, and animal experiments demonstrating antiviral potential. Applied Microbiology and Biotechnology 105 (1), pp. 129 - 146 (2021)
Journal Article
Mahour, R.; Marichal-Gallardo, P.; Rexer, T.; Reichl, U.: Multi-enzyme cascades for the in vitro synthesis of guanosine diphosphate L-fucose. ChemCatChem 13 (8), pp. 1981 - 1989 (2021)
Journal Article
Marichal-Gallardo, P.; Börner, K.; Pieler, M.; Sonntag-Buck, V.; Obr, M.; Bejarano, D.; Wolff, M. W.; Kräusslich, H.-G.; Reichl, U.; Grimm, D.: Single-use capture purification of adeno-associated viral gene transfer vectors by membrane-based steric exclusion chromatography. Human Gene Therapy 32 (17-18), pp. 959 - 974 (2021)
Journal Article
Rand, U.; Kupke, S. Y.; Shkarlet, H.; Hein, M. D.; Hirsch, T.; Marichal-Gallardo, P.; Cicin-Sain, L.; Reichl, U.; Bruder, D.: Antiviral Activity of Influenza A Virus Defective Interfering Particles against SARS-CoV-2 Replication In Vitro through Stimulation of Innate Immunity. Cells 10 (7), 1756 (2021)
2019
Journal Article
Gränicher, G.; Coronel, J.; Pralow, A.; Marichal-Gallardo, P.; Wolff, M. W.; Rapp, E.; Karlas, A.; Sandig, V.; Genzel, Y.; Reichl, U.: Efficient influenza A virus production in high cell density using the novel porcine suspension cell line PBG.PK2.1. Vaccine 37 (47), pp. 7019 - 7028 (2019)
2017
Journal Article
Marichal-Gallardo, P.; Pieler, M.; Wolff, M. W.; Reichl, U.: Steric exclusion chromatography for purification of cell culture-derived influenza A virus using regenerated cellulose membranes and polyethylene glycol. Journal of Chromatography A 1483 (3), pp. 110 - 119 (2017)

Meeting Abstract (2)

2019
Meeting Abstract
Marichal-Gallardo, P.; Genzel, Y.; Börner, K.; Grimm, D.; Wolff, M. W.; Reichl, U.: A single-use chromatographic purification platform for viral gene transfer vectors & viral vaccines. In Proceedings Advancing Manufacture of Cell and Gene Therapies VI, 92. Advancing Manufacture of Cell and Gene Therapies VI, Loews Coronado Bay Hotel Coronado, California, USA, January 27, 2019 - January 31, 2019. (2019)
2018
Meeting Abstract
Marichal-Gallardo, P.; Fortuna, A. R.; Genzel, Y.; Wolff, M. W.; Reichl, U.: Purifying viruses with a sheet of paper: Single-use steric exclusion chromatography as a capture platform for vaccine candidates. In Vaccine Technology VII, 128. Vaccine Technology VII, Mont Tremblant, Canada, June 17, 2018 - June 22, 2018. (2018)

Talk (11)

2023
Talk
Dogra, T.; Pelz, L.; Boehme, J. D.; Baelkner, M.; Kershaw, O.; Marichal-Gallardo, P.; Gruber, A. D.; Genzel, Y.; Bruder, D.; Kupke, S. Y. et al.; Reichl, U.: Genetically engineered defective interfering particles of influenza A virus for antiviral treatment and vaccination. 17th Vaccine Congress, Glasgow (Scotland) (2023)
Talk
Pelz, L.; Dogra, T.; Boehme, J.D.; Hein, M. D.; Marichal-Gallardo, P.; Genzel, Y.; Bruder, D.; Kupke, S. Y.; Reichl, U.: Influenza A virus OP7 defective interfering particles: Cell culture-based production and antiviral efficacy in vivo. Cell Culture Engineering (CCE) XVIII , Cancun (Mexico) (2023)
2022
Talk
Gränicher, G.; Göbel, S.; Marichal-Gallardo, P.; Jordan, I.; Sandig, V.; Genzel, Y.; Reichl, U.: Integrated cell-culture based MVA virus production process with perfusion mode for vaccines or gene therapy. Value through intensified bioprocessing II, Oxford, United Kingdom (2022)
Talk
Gränicher, G.; Göbel, S.; Marichal-Gallardo, P.; Jordan, I.; Sandig, V.; Genzel, Y.; Reichl, U.: Integrated cell-culture based MVA virus production process with perfusion mode for vaccines or gene therapy. 27th ESACT Meeting, Lisbon (Portugal) (2022)
Talk
Kupke, S. Y.; Hein, M.D.; Bdeir, N.; Rand, U.; Marichal-Gallardo, P.; Cicin-Sain, L.; Bruder, D.; Pöhlmann, S.; Schughart, K.; Reichl, U.: Influenza A Virus Defective Interfering Particles for Antiviral Treatment of the Influenza Disease and COVID-19. 31st Annual Meeting of the Society for Virology, online (2022)
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