Tuesday

9:00

Keynote Lecture:

Matthew DeLisa, Cornell U, Ithaca, USA

Adventures in bacterial glycobiology: engineering sweet solutions to sticky situations

Session: Synthetic Glycoengineering and Glycoengineering via Cell Line Design - Part 1

Chairs: Laura Palomares & Stefan Kubick

9:40

Tim Keys, ETH Zürich, Switzerland

GlyColi - a synthetic glycobiology platform for producing the next generation of glycoprotein therapeutics and vaccines in bacteria

10:00

Zaid Alhajajj, MLU Halle-Wittenberg, Germany

Investigations of enzymatic glycosylation in vitro using oligosaccharyltransferase from Saccharomyces cerevisiae

10:20

Ryuta Wada, Astellas Pharma, Tsukuba City, Japan

Impact of N-glycosylation on effector functions and thermal stability of monoclonal antibody with homogeneous glycoforms generated by chemoenzymatic glycoengineering approach

10:40

Lasse Pedersen, DTU, Kgs Lyngby, Denmark

High-Throughput genome engineering of CHO cells using CRISPR/Cas9

11:00

Break with Coffee & Tea

11:30

Keynote Lecture:

Geert-Jan Boons, Utrecht U & CCRC, U of Georgia, Athens, USA

Chemo-Enzymatic approaches for the preparation of highly complex glycans of biological importance

Session: Synthetic Glycoengineering and Glycoengineering via Cell Line Design - Part 2

Chairs: Laura Palomares & Stefan Kubick

12:10

Lothar Elling, RWTH Aachen, Germany

A novel enzyme module system for the one-pot synthesis of hyaluronic acid from sucrose and N-acetylglucosamine

12:30

Benjamin Schumann, Imperial College, London, UK

Understanding the molecular details of mucin-type O-glycosylation by glycosyltransferase bump-hole engineering

12:50

Fabian Pfrengle, MPI Potsdam, Germany

Characterizing plant glycosyltransferases in high-throughput using azido-functionalized sugar nucleotides on glycan arrays

13:10

Lunch

14:30

Rico Uhler, Mannheim U of Applied Science, Germany

Glycoengineering of the human embryonic kidney FreeStyle 293-F cell line towards prolongend bioavailablity of recombinant coagulation factor VII

14:50

Keynote Lecture:

Xi Chen, U of California, Davis, USA

One-pot multienzyme (OPME) chemoenzymatic synthesis of complex carbohydrates

Session: Synthesis of Oligossaccharides, Glycans and Nucleotide Sugar

Chair: Lothar Elling

15:30

Nathan Lewis, U of California, San Diego, USA

Elucidating the biosynthetic pathways of human milk oligosaccharides through a systems biology approach

15:50

Reza Mahour, MPI Magdeburg, Germany

Multi-enzyme process development for production of glycosyltransferase substrates: activated sugars

16:10

Anne Usvalampi, Aalto U, Espoo, Finland

Exploring transglycosylation reactions for production of novel fucose-containing oligosaccharides

16:30

Rachel Chen, Georgia Institute of Technology, Atlanta, USA

Novel metabolic engineering strategies for oligosaccharides synthesis

16:50

Break with Coffee & Tea

Session: Impact of Expression Systems and Cultivation Conditions on Glycosylation

Chair: Alexander Frey

17:20

Laura Palomares, UNAM, Cuernavaca, Mexico

Growth rate of CHO cells cultures in perfusion cultures determines the quality of a monoclonal antibody

17:40

Seongkyu Yoon, U of Massachusetts Lowell, USA

An Illumination of Nucleotide Sugar Dynamics Under the Impact of Galactose Supplementation in CHO Cell Culture

18:00

Sumit Madhuresh, Pfizer Inc, Andover, USA

Novel mechanistic insights towards precision control of N-glycosylation in CHO cell fed-batch cultures

18:20

Dine&Discuss – Food, beer, wine and soft drinks are included

 From 20:30 the Einstein Lounge is open and free drinks are served.

 

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