Tuesday
9:00 |
Keynote Lecture: Matthew DeLisa, Cornell U, Ithaca, USAAdventures in bacterial glycobiology: engineering sweet solutions to sticky situations |
Session: Synthetic Glycoengineering and Glycoengineering via Cell Line Design - Part 1
Chairs: Laura Palomares & Stefan Kubick |
|
9:40 |
Tim Keys, ETH Zürich, Switzerland GlyColi - a synthetic glycobiology platform for producing the next generation of glycoprotein therapeutics and vaccines in bacteria |
10:00 |
Zaid Alhajajj, MLU Halle-Wittenberg, Germany Investigations of enzymatic glycosylation in vitro using oligosaccharyltransferase from Saccharomyces cerevisiae |
10:20 |
Ryuta Wada, Astellas Pharma, Tsukuba City, Japan Impact of N-glycosylation on effector functions and thermal stability of monoclonal antibody with homogeneous glycoforms generated by chemoenzymatic glycoengineering approach |
10:40 |
Lasse Pedersen, DTU, Kgs Lyngby, Denmark High-Throughput genome engineering of CHO cells using CRISPR/Cas9 |
11:00 |
Break with Coffee & Tea |
11:30 |
Keynote Lecture: Geert-Jan Boons, Utrecht U & CCRC, U of Georgia, Athens, USA Chemo-Enzymatic approaches for the preparation of highly complex glycans of biological importance |
Session: Synthetic Glycoengineering and Glycoengineering via Cell Line Design - Part 2
Chairs: Laura Palomares & Stefan Kubick |
|
12:10 |
Lothar Elling, RWTH Aachen, Germany A novel enzyme module system for the one-pot synthesis of hyaluronic acid from sucrose and N-acetylglucosamine |
12:30 |
Benjamin Schumann, Imperial College, London, UK Understanding the molecular details of mucin-type O-glycosylation by glycosyltransferase bump-hole engineering |
12:50 |
Fabian Pfrengle, MPI Potsdam, Germany Characterizing plant glycosyltransferases in high-throughput using azido-functionalized sugar nucleotides on glycan arrays |
13:10 |
Lunch |
14:30 |
Rico Uhler, Mannheim U of Applied Science, Germany Glycoengineering of the human embryonic kidney FreeStyle 293-F cell line towards prolongend bioavailablity of recombinant coagulation factor VII |
14:50 |
Keynote Lecture: Xi Chen, U of California, Davis, USA One-pot multienzyme (OPME) chemoenzymatic synthesis of complex carbohydrates |
Session: Synthesis of Oligossaccharides, Glycans and Nucleotide SugarChair: Lothar Elling |
|
15:30 |
Nathan Lewis, U of California, San Diego, USA Elucidating the biosynthetic pathways of human milk oligosaccharides through a systems biology approach |
15:50 |
Reza Mahour, MPI Magdeburg, Germany Multi-enzyme process development for production of glycosyltransferase substrates: activated sugars |
16:10 |
Anne Usvalampi, Aalto U, Espoo, Finland Exploring transglycosylation reactions for production of novel fucose-containing oligosaccharides |
16:30 |
Rachel Chen, Georgia Institute of Technology, Atlanta, USA Novel metabolic engineering strategies for oligosaccharides synthesis |
16:50 |
Break with Coffee & Tea |
Session: Impact of Expression Systems and Cultivation Conditions on Glycosylation
Chair: Alexander Frey |
|
17:20 |
Laura Palomares, UNAM, Cuernavaca, Mexico Growth rate of CHO cells cultures in perfusion cultures determines the quality of a monoclonal antibody |
17:40 |
Seongkyu Yoon, U of Massachusetts Lowell, USA An Illumination of Nucleotide Sugar Dynamics Under the Impact of Galactose Supplementation in CHO Cell Culture |
18:00 |
Sumit Madhuresh, Pfizer Inc, Andover, USA Novel mechanistic insights towards precision control of N-glycosylation in CHO cell fed-batch cultures |
18:20 |
Dine&Discuss – Food, beer, wine and soft drinks are included |
From 20:30 the Einstein Lounge is open and free drinks are served.