Programme
Day 1
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8:15 |
Registration, Check-In, Coffee&Tea |
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9:15 |
Welcome and opening remarks |
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9:30 |
Keynote Lecture: Markus Aebi, ETH Zurich, Switzerland Freedom to operate: concepts of N-linked protein glycosylation |
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Session: Glycosylation & Protein FunctionChair: Niclas Karlsson and Rita Gerardy-Schahn |
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10:10 |
Elisa Benedetti, Helmholtz Center Munich, Germany Network inference from glycoproteomics data reveals new reactions in the IgG glycosylation pathway |
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10:30 |
Gordan Lauc, University of Zagreb, Croatia Functional relevance of inter-individual differences in the IgG glycome |
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10:50 |
Anna-Janina Behrens, University of Oxford, United Kingdom Site-specific N-glycosylation analysis of the HIV-1 glycan shield |
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11:10 |
Break – Coffee&Tea, Pastries, Fruits |
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11:40 |
Serge Perez, CERMAV-CNRS, University of Grenoble Alpes, France Dynamics of fucose and Lewis x in lectin-binding sites |
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12:00 |
Katrine Schjoldager, Copenhagen Center for Glycomics, Denmark Site-specific O-glycosylation modulates HDL-metabolism in vivo |
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12:20 |
Jens Kopatz, University Hospital Bonn, Germany Low molecular weight polysialic acid inhibits the innate immune system and is a promising candidate for therapy of age related macular degeneration |
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12:40 |
Rob Field, John Innes Centre, United Kingdom Dissecting plant polysaccharide biosynthesis |
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13:00 |
Lunch Break |
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14:15 |
Invited Lecture: Dietmar Reusch, F. Hoffmann - La Roche AG, Germany Comparison of methods for IgG glycosylation - a multi laboratory study |
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Session: Technologies & Methods for GlycoanalysisChair: Erdmann Rapp |
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14:50 |
Alexandra Stuckmann, Free University Berlin, Germany Ion mobility-mass spectrometry of isomeric glycans – the impact of reducing end modifications |
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15:10 |
Kathrin Stavenhagen, VU University Amsterdam, The Netherlands Site-specific N- and O-glycosylation analysis of antibody-based therapeutics |
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15:30 |
Monique van Scherpenzeel, Radboudumc, The Netherlands Sensitive ion pair UHPLC-QqQ to analyze the building blocks of glycosylation gives new mechanistic insights and opportunities for therapy supplementation in congenital disorders of glycosylation |
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15:50 |
Break – Coffee&Tea, Pastries, Fruits |
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16:20 |
Radoslaw Kozak, Ludger Ltd., United Kingdom Analysis of biopharmaceutical Glycosylation Critical Quality Attributes (GCQAs) |
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16:40 |
Jean Dubayle, Sanofi Pasteur, France Advances in glycosylation characterization of vaccine antigens |
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17:00 |
Mario Schubert, University Salzburg, Austria Characterizing glycans in intact proteins by NMR spectroscopy |
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17:20 |
Jun Hirabayashi, AIST, Japan Development of lectin-based glycan profiling technologies: reinforced frontal affinity chromatography and evanescent wave-activated lectin microarray |
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17:40 |
Falk Buettner, Hannover Medical School, Germany Glycoproteomic analysis of human pluripotent stem cells and stem cell-derived cardiomyocytes |
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18:00 |
Dine&Discuss – Food, beer, wine and soft drinks are included |
Day 2
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9:00 |
Keynote Lecture: Nico Callewaert, VIB Medical Biotechnology Center, Belgium Customization of biopharmaceutical protein glycosylation |
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Session: Glycoengineering via Cell Line DesignChair: Nathan Lewis and Alexander Frey |
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9:40 |
Nathan Lewis, University of California, San Diego, United States A systems approach to cell line selection and design for rational glycoengineering |
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10:00 |
Alexander Frey, Aalto University, Finland Impact of glycoengineering on glycosylation efficiency, cellular quality control systems and product quality |
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10:20 |
Robert Woods, CCRC, University of Georgia, United States Predicting N-glycan processing based on enzyme-glycan accessibility |
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10:40 |
Break – Coffee&Tea, Pastries, Fruits |
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11:10 |
Invited Lecture: Henrik Clausen, Copenhagen Center for Glycomics, Denmark Stable glycoengineering of mammalian cells |
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11:45 |
Niklas Karlsson, University of Gothenburg, Sweden Glycomodified Chinese Hamster Ovary cells to investigate glycosyltransferase specificity and function of O-linked glycans |
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12:05 |
Stephan Hinderlich, Beuth University of Applied Sciences Berlin, Germany Engineering of CHO cells for the production of recombinant glycoproteins with xylosylated N-glycans |
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12:25 |
Lennard Bohlender, University of Freiburg, Germany Production of biologically active recombinant factor H in glyco-optimized Physcomitrella patens |
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12:45 |
Lunch Break |
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14:05 |
Sabine Flitsch, University of Manchester, United Kingdom Metrology of carbohydrates for enabling European bioIndustries |
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14:10 |
Keynote Lecture: Michael J. Betenbaugh, John Hopkins University, United States Looking inside and out: Systems approaches to glycoengineering of mammalian bioprocesses |
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Session: Synthetic & Biosynthetic GlycoengineeringChair: Lothar Elling and Markus Pietzsch |
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14:50 |
Marco Thomann, Roche Diagnostics GmbH, Germany In vitro glycoengineering – A useful tool for protein characterization and optimization through modification of the glycosylation pattern |
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15:10 |
Lothar Elling, Helmholtz Institute for Biomedical Engineering RWTH Aachen, Germany Multivalent neo-glycoproteins as specific galectin ligands and serum diagnostics |
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15:30 |
Anna Schildbach, MLU Halle-Wittenberg, Germany In-vitro synthesis of phytanyl-pyrophosphate-linked oligosaccharides by cascades of glycosyltransferases as basic platform of N-glycosylation of therapeutic proteins |
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15:50 |
Break – Coffee&Tea, Pastries, Fruits |
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16:20 |
Jonathan Sjörgen, Genovis AB, Sweden Site specific and quantitative conjugation of functional groups to antibody N-glycans using the novel GlyCLICK™ technology |
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16:40 |
Peng George Wang, Georgia State University, United States Enzymatic synthesis and bioengineering for synthetic glycomes |
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17:00 |
Timm Fiebig, Medical School Hannover, Germany An enzyme-based synthesis of a meningococcal glycoconjugate vaccine |
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17:20 |
Anne Imberty, CERMAV-CNRS, University of Grenoble Alpes, France New tools from synthetic glycobiology: Engineering lectins valency, architecture and specificity |
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17:40 |
Daniel Varon Silva, Max Planck Institute of Colloids and Interfaces, Germany Protein semi-synthesis as tool to disclose the role of protein glypiation |
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18:00 |
Dine&Discuss – Food, beer, wine and soft drinks are included |
Day 3
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9:00 |
Invited Lecture: Michael Butler, NIBRT, Ireland The influence of cultivation conditions on glycosylation |
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Session: Impact of Expression Systems & Cultivation Conditions on GlycosylationChair: Udo Reichl |
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9:35 |
David Bruehlmann, Merck Biopharma, Switzerland The Potential of small molecules to modulate glycosylation by media design |
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9:55 |
Lars Stoeckl, Glycotope GmbH, Germany A toolbox of human cell lines for the screening and production of fully human and glycooptimized biotherapeutics |
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10:15 |
Break – Coffee&Tea, Pastries, Fruits |
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10:35 |
Ioscani Jimenez del Val, University College Dublin, Ireland A metabolic flux model that quantifies the partition of resources between cellular and recombinant protein glycosylation in GS-CHO cells |
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10:55 |
Stefan Kubick, Fraunhofer Institute for Cell Therapy and Immunology, Germany Cell-free synthesis of glycoproteins |
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11:15 |
Closing remarks |
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11:30 |
End of the symposium |