Team Leader (USP)

PD Dr. Yvonne Genzel
PD Dr. Yvonne Genzel
Phone: +49 391 6110 257
Room: N0.18

Additional Information

Internal Collaborations:

F. Tapia, Dipl. Chem Eng; M. Sc. Upstream Processing, BPE, MPI Magdeburg

Dipl.-Ing. M. Pieler. Downstream Processing, BPE, MPI Magdeburg

P. Marichal-Gallardo, M. Sc. Downstream Processing, BPE, MPI Magdeburg

External Collaborations:

Dr. Rui Oliveira, Faculty of Sciences and Technology, Universidade Nova de Lisboa, Portugal

Dr. Moritz von Stosch, CEAM, Newcastle University, United Kingdom

Dr. Ingo Jordan, Dr. Volker Sandig, ProBioGen AG, Berlin, Germany

Dr. Laura A. Palomares, Instituto de Biotecnología, UNAM, Mexico

Production of Viral Vaccines in High-Cell-Density Suspension Cultures

Production of Viral Vaccines in High-Cell-Density Suspension Cultures


Process intensification for the production of various animal cell culture-based biopharmaceuticals is currently carried out through high-cell-density (HCD) cultivations. Cultivations in perfusion mode are commonly applied for manufacturing of recombinant proteins [1]. Production of viral vaccines under HCD conditions, however, has been little explored in large scale production, and often a significant decrease in cell-specific yields (virus/cell) and/or volumetric productivity (virus/L·d) compared to conventional batch processes has been observed [2]. Furthermore, due to virus-induced apoptosis and cell death, continuous virus production strategies in perfusion mode are challenging. Nevertheless, to increase virus yields, advanced process strategies suitable to overcome the intrinsic challenges of virus propagation in HCD cultures should be established and optimized.

Aims of the project

  • Design and optimization of a perfusion-based process for the cultivaton of suspension cells at HCD using alternating tangential flow (ATF) and tangential flow filtration (TFF) systems.
  • Monitoring of critical state variables to characterize cell growth and virus replication dynamics.
  • Analysis of the impact of medium composition on virus quality and process yields.
  • Establishment of mathematical models for control of cultivation conditions to increase process productivity.
  • Identification of critical parameters for integration of upstream and downstream operations.
<em>Experimental setup for a perfusion-based process using an ATF system.</em> Zoom Image
Experimental setup for a perfusion-based process using an ATF system.


[1] Kompala, D.S. and S.S. Ozturk, Optimization of high cell density perfusion bioreactors. 2006: Taylor & Francis: New York.

[2] Genzel, Y., et al., High cell density cultivations by alternating tangential flow (ATF) perfusion for influenza A virus production using suspension cells. Vaccine, 2014. 32(24): p. 2770-81.

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