Team Leader (USP)

PD Dr. Yvonne Genzel
PD Dr. Yvonne Genzel
Phone: +49 391 6110 257

Researcher

Alexander Nikolay, M. Sc.
Alexander Nikolay, M. Sc.
Phone: +49 391 6110 226

Additional Information

External collaborations

University of Applied Science Emden/Leer, Institute of Bioprocess Engineering
(Prof. Dr. K. Scharfenberg)

Federal University of Rio de Janeiro, Cell Culture Engineering Laboratory, Brazil
(Prof. Dr. Leda Castilho)

Internal collaborations

Bio/Process Analytics
(T. Nguyen-Khuong, R. Hennig)

Downstream Processing
(M. Pieler, R. Fortuna)

Yellow Fever Vaccine Production

Yellow Fever Vaccine Production

Motivation

Yellow fever virus as recombinant vector can be used to design vaccines against Dengue fever, West Nile fever, Japanese encephalitis, and other serious diseases. If such a disease emerges rapidly among humans, quick vaccination is crucial to control viral spreading. Current manufacturers produce yellow fever vaccines in chicken embryos, a slow and elaborate method essentially unchanged over the past 60 years. Moving towards cell culture-based production approaches in bioreactors could overcome limitations of current manufacturing processes, and support the production of affordable high quality vaccines [1].

Aim of the project

We investigate novel and efficient scale-up options for yellow fever vaccine production. This virus is considered as a model system for the production of chimeric vector strains designed against genus-related diseases, i.e. West Nile and Japanese encephalitis. The focus of this work is on the identification of suitable cell substrates, suspension growth in serum-free media, and process intensification in bioreactors. Obtained cell lines and optimized laboratory scale processes will be assessed by time-specific virus yields [2]. In addition, viral antigens will be characterized by analyzing their N-glycosylation fingerprints.

<em>(Left Figure) Stirred tank bioreactor with suspension cells at 5&nbsp;L scale. (Right Figure) Cell growth dynamics and Yellow Fever Virus yields of three different adherent cell lines to identify the best cell substrate for vaccine manufacturing.</em> Zoom Image
(Left Figure) Stirred tank bioreactor with suspension cells at 5 L scale. (Right Figure) Cell growth dynamics and Yellow Fever Virus yields of three different adherent cell lines to identify the best cell substrate for vaccine manufacturing. [less]

References

1.
Gallo Ramirez, L. E.; Nikolay, A.; Genzel, Y.; Reichl, U.: Bioreactor concepts for cell culture-based viral vaccine production. Expert Review of Vaccines 14 (9), pp. 1181 - 1191 (2015)
2.
Nikolay, A.; Scharfenberg, K.; Patel, P.; Niedrig, M.; Genzel, Y.; Reichl, U.: Towards yellow fever 17D virus production in Vero suspension cells. ESACT, Barcelona (2015)
3.
Nikolay, A.; Casthilho, L. R.; Reichl, U.; Genzel, Y.: Propagation of Brazilian Zika virus strains in static and suspension cultures using Vero and BHK cells. Vaccine (accepted)
 
loading content