Yellow Fever Vaccine Production
Yellow fever virus as recombinant vector can be used to design vaccines against Dengue fever, West Nile fever, Japanese encephalitis, and other serious diseases. If such a disease emerges rapidly among humans, quick vaccination is crucial to control viral spreading. Current manufacturers produce yellow fever vaccines in chicken embryos, a slow and elaborate method essentially unchanged over the past 60 years. Moving towards cell culture-based production approaches in bioreactors could overcome limitations of current manufacturing processes, and support the production of affordable high quality vaccines .
Aim of the project
We investigate novel and efficient scale-up options for yellow fever vaccine production. This virus is considered as a model system for the production of chimeric vector strains designed against genus-related diseases, i.e. West Nile and Japanese encephalitis. The focus of this work is on the identification of suitable cell substrates, suspension growth in serum-free media, and process intensification in bioreactors. Obtained cell lines and optimized laboratory scale processes will be assessed by time-specific virus yields . In addition, viral antigens will be characterized by analyzing their N-glycosylation fingerprints.