Team Leader (DSP)

Prof. Dr. Michael Wolff
Prof. Dr. Michael Wolff
Phone: +49 391 67 546 76
Fax: +49 391 6110 588
Room: G25-114

Magnetic Particles for Virus Purification

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Development of Magnetic Particles for Virus Purification Processes

Motivation

Viruses can be purified by numerous methods like centrifugation, filtration, or chromatography. However, these methods are usually not intended for small scale purification or virus isolation. Furthermore, they are not suited for high-throughput isolations or analysis of patient or environmental samples.
Separation via magnetic particles, is a promising alternative [1]. Classically, magnetic particle separation is based on antibodies immobilized onto magnetic beads, allowing a specific isolation of target (macro-)molecules up to cells. However, use of the method requires specific antibodies for every target which increases costs and narrows down its field of applications. Both disadvantages can be circumvented, by using pseudo-affinity ligands based on small molecules, like sulfated carbohydrates, which are applicable for a wide range of targets [2],[3].

Figure 1. Magnetic sulfated cellulose particles (MSCP) Zoom Image
Figure 1. Magnetic sulfated cellulose particles (MSCP)

Aim of the project

Combining the advantages of pseudo-affinity ligands with magnetic separation, we develop magnetic sulfated cellulose particles (MSCP) to purify or deplete a variety of viruses including influenza A and B virus and modified Vaccinia Ankara (MVA) virus [4],[5].

References

[1] L. Borlido, A. M. Azevedo, A. C. A. Roque, and M. R. Aires-Barros, ‘Magnetic separations in biotechnology’, Biotechnol. Adv., vol. 31, no. 8, pp. 1374–1385, Dec. 2013.
[2] P. F. O’neil and E. S. Balkovic, ‘Virus harvesting and affinity-based liquid chromatography’, Bio/technology, vol. 11, no. 2, pp. 173–178, 1993.
[3] M. W. Wolff, C. Siewert, S. P. Hansen, R. Faber, and U. Reichl, ‘Purification of cell culture-derived modified vaccinia ankara virus by pseudo-affinity membrane adsorbers and hydrophobic interaction chromatography’, Biotechnol. Bioeng., vol. 107, no. 2, pp. 312–320, 2010.
[4] M. Wolff, M. M. Pieler, and U. Reichl, ‘Process for the preparation of magnetic sulfated cellulose particles, magnetic sulfated cellulose particles and its use’, EP14175925. (pending)
[5] A. Serve, M. M. Pieler, D. Benndorf, E. Rapp, M. W. Wolff, and U. Reichl, ‘Comparison of Influenza Virus Particle Purification Using Magnetic Sulfated Cellulose Particles with an Established Centrifugation Method for Analytics’, Anal. Chem., Oct. 2015.

 
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