Master Thesis (m/f/d) | Optimization of the purification of glycans

Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg

Type of Job

Young Researchers

Structural and Cell Biology Chemistry Complex Systems

Job offer from November 04, 2022

In vitro glycoengineering offers the possibility to modify the glycosylation of glycoproteins and thus to produce specific glycan structures as desired. In the Synthetic Biotechnology group, Dept. of Bioprocess Engineering, of the Max Planck Institute for Dynamics of Complex Technical Systems enzymatic processes for in vitro glycosylation are developed. The substrate of the enzymes consists of glycans, which are present in a reaction mix. Within the scope of a master thesis, a purification method should be optimized to separate and purify different glycans. With work packages in the areas of biocatalysis and downstream processing, as well as in the areas of expression of recombinant enzymes and reaction analysis, the master project is very interdisciplinary. We are looking for a motivated individual (m/f/d) with very good basic knowledge in the field of biotechnology and chemistry/biochemistry as well as with interest in the development of biocatalytic methods to work on the project.

The focus of the project is on:

  • Purification of glycans
  • Glycoanalysis by HILIC and capillary electrophoresis
  • Recombinant production of proteins in microbial systems
  • Downstream processing of recombinant enzymes by chromatography and filtration methods

Candidates will have the opportunity to work on key technologies in an international research group with state-of-the-art equipment. In addition, thorough supervision and training by MPI staff will be provided.

The start date is planned for January/Feburary 2023.

You bring along

  • Degree: biotechnology, bioprocess engineering, biochemistry
  • Experience with laboratory work and ideally experience with one of the following techniques: recombinant protein expression, chromatography methods, downstream processing, extraction methods of lipids
  • Motivation, curiosity and ability to work in a team

We offer

  • An exciting research project and the opportunity to develop key technologies
  • First-class supervision and training by our staff members
  • Acquisition of knowledge in the fields of biocatalysis, recombinant enzyme expression, analytics by ion chromatography and downstream processing
  • Familiarization with state-of-the-art equipment for biotechnological research and development

Application

Please send your application with CV and references in German or English to one of the following contacts:

Johannes Ruhnau, M.Sc.
Email:
Phone: +49 (0) 391 6110 371

Lisa Wenzel, M.Sc.
E-mail:
Phone: +49 (0) 391 6110 229

The Max Planck Society strives for gender equity and diversity. We welcome applications of any background. Furthermore, the Max Planck Society has set itself the goal of employing more severely disabled people. Applications from severely disabled people are explicitly encouraged.

Please note the information regarding storage of personal data.

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