Method Development for Multiplexing CGE-LIF Based Glycoanalysis
Analysis of Virus-Host Cell Interaction on the Proteome Level
This project was related to other genomic and metabolic approaches, all focusing on the dynamic interaction of viruses with their mammalian host cells during vaccine production. Therefore, changes in the expression of host cell proteins in various mammalian cells after infection with different virus strains were characterized. In addition, proteome changes related to cultivation conditions were investigated.
Aim of the project
Using two dimensional differential gel electrophoresis and mass spectrometry (Figure 1) we identified differentially expressed host cell proteins over the course of an infection phase. Different cell lines, like Vero and MDCK cells, are hosts for cell-based vaccine production. The results were correlated with integrated process data and gave detailed insight into vaccine production on the cellular level. This knowledge contributed to a better characterization und understanding of the complexity of virus-host cell interactions.