Yvonne Genzel

MPI Magdeburg

Address:


PD Dr. Yvonne Genzel
Max-Planck-Institute for Dynamics of Complex Technical Systems
Sandtorstrasse 1
D-39106 Magdeburg
Germany

Phone:

+49 (0)391 6110-257

Fax:

+49 (0)391 6110-565

E-mail:

genzel@mpi-magdeburg.mpg.de

 

 

 

Brief biography

- Born 1972 in Friedrichshafen (Germany)

- Lived from 1974-1991 in Noordwijk (The Netherlands)

- Studies of Technical Biology from 1991-1997 at the University of Stuttgart (Germany)

- Diploma Thesis "Purification and characterisation of an epoxide hydrolase from Nocardia sp. EH1" at the Institute for Biochemical Engineering, University of

Stuttgart, Germany (Prof. C. Syldatk) IBVT Stuttgart in cooperation with Prof. K. Faber, University of Graz, Austria. University Graz

- From 1997-2000: Ph.D. Thesis in the "Biocatalysis and Chemistry" group (CNRS) of Dr. R. Furstoss at the University of the Mediterranean, Marseille, France.

"Preparation of enantiopure 2-, 3- and 4-pyridyloxirane via epoxide hydrolase catalyzed hydrolytic kinetic resolution"

- since January 2001 member of the Bioprocess Engineering group at the Max-Planck-Institute for Dynamics of Complex Technical Systems, responsible for the upstream processing group.

- 2009 Habilitation "Upstream processing issues in influenza vaccine production using animal cell technology" at the "Fakultät für Verfahrens- und Systemtechnik" Otto-von-Guericke-Universität Magdeburg.

 

Research Interests

Awards

·         2007 Cologne Bioinnovation Award 2007 - best poster at the European Bioperspectives 2007- "Vero or MDCK cells for influenza A virus production in microcarrier systems?" C. Dietzsch, Y. Genzel, U. Reichl

Fellowships

·         1997-2000 Fellowship from DSM (The Netherlands)

·         1998 Procope project between Germany and France: 1 month guest researcher at the Institute for Biotechnology II, Forschungszentrum Jülich, Germany

"Optimization of reaction conditions by a genetic algorithm for the bioconversion of p-Cl-styrene oxide and 2-pyridyloxirane by enzymatic extract of A. niger LCP48.521" biotechnology in Jülich

Publications

·         Roedig JV, Rapp E, Genzel Y, Reichl U." Impact of different influenza cultivation conditions on HA N-Glycosylation." BMC Proc. ,2011 ;5 Suppl 8:P113.

·         Janke R, Genzel Y, Wetzel M, Reichl U." Effect of influenza virus infection on key metabolic enzyme activities in MDCK cells." BMC Proc. ,2011 ;5 Suppl 8:P129.

·         Roedig JV, Rapp E, Höper D, Genzel Y, Reichl U." Impact of host cell line adaptation on quasispecies composition and glycosylation of influenza a virus hemagglutinin." PLoS One. , 2011;6(12):e27989.

·         Janke R, Genzel Y, Händel N, Wahl A, Reichl U." Metabolic adaptation of MDCK cells to different growth conditions: effects on catalytic activities of central metabolic enzymes." Biotechnol Bioeng. , 2011;108(11):2691-704.

·         Kalmbach A, Bordás R, Oncül AA, Thévenin D, Genzel Y, Reichl U." Experimental characterization of flow conditions in 2- and 20-L bioreactors with wave-induced motion." Biotechnol Prog. , 2011;27(2):402-9.

·         Bock A, Schulze-Horsel J, Schwarzer J, Rapp E, Genzel Y, Reichl U." High-density microcarrier cell cultures for influenza virus production. "Biotechnol Prog. ,2011 ,27(1):241-50.

·         Janke R, Genzel Y, Freund S, Wolff MW, Grammel H, Rühmkorf C, Seidemann J, Wahl A, Reichl U." Expression, purification, and characterization of a His6-tagged glycerokinase from Pichia farinosa for enzymatic cycling assays in mammalian cells. "J Biotechnol. ,2010 ,150(3):396-403.

·         Heynisch B, Frensing T, Heinze K, Seitz C, Genzel Y, Reichl U." Differential activation of host cell signalling pathways through infection with two variants of influenza A/Puerto Rico/8/34 (H1N1) in MDCK cells. "Vaccine. ,2010 ,28(51):8210-8.

·         Lohr V, Genzel Y, Behrendt I, Scharfenberg K, Reichl U." A new MDCK suspension line cultivated in a fully defined medium in stirred-tank and wave bioreactor. "Vaccine. , 2010, 28(38):6256-64.

·         Genzel Y, Dietzsch C, Rapp E, Schwarzer J, Reichl U." MDCK and Vero cells for influenza virus vaccine production: a one-to-one comparison up to lab-scale bioreactor cultivation. "Appl Microbiol Biotechnol. ,2010 ,88(2):461-75.

·         Janke R, Genzel Y, Wahl A, Reichl U." Measurement of key metabolic enzyme activities in mammalian cells using rapid and sensitive microplate-based assays. "Biotechnol Bioeng. ,2010 ,107(3):566-81.

·         Ritter JB, Wahl AS, Freund S, Genzel Y, Reichl U." Metabolic effects of influenza virus infection in cultured animal cells: Intra- and extracellular metabolite profiling. "BMC Syst Biol. ,2010, 4:61.

·         Vester D, Rapp E, Kluge S, Genzel Y, Reichl U." Virus-host cell interactions in vaccine production cell lines infected with different human influenza A virus variants: a proteomic approach. "J Proteomics. ,2010 ,73(9):1656-69.

·         Vester D, Lagoda A, Hoffmann D, Seitz C, Heldt S, Bettenbrock K, Genzel Y, Reichl U." Real-time RT-qPCR assay for the analysis of human influenza A virus transcription and replication dynamics. "J Virol Methods. ,2010 ,168(1-2):63-71.

·         Oncül AA, Kalmbach A, Genzel Y, Reichl U, Thévenin D." Characterization of flow conditions in 2 L and 20 L wave bioreactors using computational fluid dynamics. ", Biotechnol Prog. ,2010 ,26(1):101-10.

·         Wahl, A.; Sidorenko, Y.; Dauner, M.; Genzel, Y.; Reichl, U.; "Metabolic flux distribution during growth of adherent growing MDCK cells in different media" in Proceedings from the 20th ESACT meeting: Cells & Culture in June 2007, Dresden, Germany. Eds. T. Noll; Springer. 2010, pp. 459.

·         Schulze-Horsel, J.; Schulze, M.; Genzel, Y.; Reichl, U.; "Single-cell approach in influenza vaccine production: apoptosis and virus production" in Proceedings from the 20th ESACT meeting: Cells & Culture in June 2007, Dresden, Germany. Eds. T. Noll; Springer. 2010, pp. 25.

·         Ritter, J. B.; Genzel, Y.; Reichl, U.; "Metabolite Analysis in Mammalian Cells : How to generate reliable data sets? " in Proceedings from the 20th ESACT meeting: Cells & Culture in June 2007, Dresden, Germany. Eds. T. Noll; Springer. 2010, pp. 397.

·         Vester, D.; Rapp, E.; Genzel, Y.; Gade, D.; Reichl, U.; "Proteomic analysis of influenza A virus infected mammalian cells by 2D-DIGE " in Proceedings from the 20th ESACT meeting: Cells & Culture in June 2007, Dresden, Germany. Eds. T. Noll; Springer. 2010, pp. 429.

·         Bock, A.; Genzel, Y.; Reichl, U.; "Monitoring of cell activity : online oxygen uptake rates in pulsed aerated cell culture" in Proceedings from the 20th ESACT meeting: Cells & Culture in June 2007, Dresden, Germany. Eds. T. Noll; Springer. 2010, pp. 391.

·         Genzel, Y.; Dietzsch, C.; Reichl, U.; "Comparing Vero and MDCK cells for influenza A virus production in microcarrier systems" in Proceedings from the 20th ESACT meeting: Cells & Culture in June 2007, Dresden, Germany. Eds. T. Noll; Springer. 2010, pp. 635.

·         Genzel Y.; Reichl U.; " Continuous cell lines as a production system for influenza vaccines. " , Exp. Rev. Vacc. , 2009, 8(12):1681-92.

·         Vester D, Rapp E, Gade D, Genzel Y, Reichl U.; " Quantitative analysis of cellular proteome alterations in human influenza A virus-infected mammalian cell lines. " , Proteomics , 2009, 9(12):3316-27.

·         Schwarzer J, Rapp E, Hennig R, Genzel Y, Jordan I, Sandig V, Reichl U.; " Glycan analysis in cell culture-based influenza vaccine production: influence of host cell line and virus strain on the glycosylation pattern of viral hemagglutinin. " , Vaccines , 2009, 27(32):4325-36.

·         Bock A, Sann H, Schulze-Horsel J, Genzel Y, Reichl U, Möhler L.; " Growth behavior of number distributed adherent MDCK cells for optimization in microcarrier cultures. " , Biotechnol. Progr. , 2009, 25(6):1717-1731.

·         Schulze-Horsel, J.; Schulze, M.; Agalaridis, G.; Genzel, Y.; Reichl, U.; " Infection dynamics and virus-induced apoptosis in cell culture-based influenza vaccine production – Flow cytometry and mathematical modeling " , Vaccine , 2009, 27(20), 2712-2722.

·         Lohr, V.; Rath, A.; Genzel, Y.; Jordan, I.; Sandig, V.; Reichl, U.; " New avian suspension cell lines provide production of influenza virus and MVA in serum-free media : studies on growth, metabolism and virus propagation " , Vaccine , 2009, 27(37), 4975-4982.

·         Schulze-Horsel, J.; Genzel, Y.; Reichl, U.; " Flow cytometric monitoring of influenza A virus infection in MDCK cells during vaccine production " , BMC Biotechnol. , 2008, 8(45).

·         Opitz, L.; Zimmermann, A.; Lehmann, S.; Genzel, Y.; Lübben, H.; Reichl, U.; Wolff, M. W.; " Capture of cell culture-derived influenza virus by lectins : strain independent, but host cell dependent " , J. Virol. Meth. , 2008, 154(1-2), 61-68.

·         Wahl, A.; Sidorenko, Y.; Dauner, M.; Genzel, Y.; Reichl, U.; " Metabolic flux model for an anchorage-dependent MDCK cell line : Characteristic Growth Phases and Minimum Substrate Consumption Flux Distribution " , Biotechnol. Bioeng. , 2008, 101(1), 135-155.

·         Sidorenko Y.; Wahl A.; Dauner M.; Genzel Y.; Reichl U.; " Comparison of Metabolic Flux Distributions for MDCK Cell Growth in Glutamine-Containing and Pyruvate-Containing Medium " , Biotechnol. Progr. , 2008, 24(2), 311-320.

·         Ritter J.B.; Genzel Y.; Reichl U.; " Simultaneous extraction of several metabolites of energy metabolism and related substances in mammalian cells: Optimization using experimtal design " , Anal. Biochem. , 2008, 373, 349-369.

·         Hundt B.; Best C.; Schlawin N.; Kaßner H.; Genzel Y.; Reichl U.; " Establishment of a Mink Enteritis Vaccine Production Process in Stirred-Tank Reactor and Wave® Bioreactor Microcarrier Culture in 1 - 10 L scale" , Vaccine , 2007, 25(20), 3987-3995.

·         Kalbfuss B.; Genzel Y.; Wolff M.; Zimmermann A.; Morenweiser R.; Reichl U.; " Harvesting and concentration of human influenza A virus produced in serum-free mammalian cell culture for the production of vaccines. " , Biotechnol. Bioeng. , 2007, 97(1), 73-85.

·         Genzel Y.; Reichl U.; "Vaccine production - state of the art and future needs in upstream processing " in Methods in Biotechnology: Animal Cell Biotechnology - Methods and Protocols ; Eds. R. Pörtner; HUMANA Press Inc., Totowa, New Jersey, 2007, 457-473.

·         Genzel, Y.; Fischer, M.; Olmer, R. M.; Schäfer, B.; Best, C.; König, S.; Hundt, B.; Reichl, U.; "Serum-free influenza vaccine production with MDCK cells in wave-bioreactor and 5L-stirred tank bioreactor" in Proceedings from the 19th ESACT meeting: Cell technology for cell products in June 2005, Harrogate, UK. Eds. R. Smith; Springer. 2007, 563-565.

·         Schulze-Horsel, J.; Genzel, Y.; Reichl, U.; "Monitoring of Cell Physiology in Influenza Vaccine Production by Flow Cytometry " in Proceedings from the 19th ESACT meeting: Cell technology for cell products in June 2005, Harrogate, UK. Eds. R. Smith; Springer. 2007, 531-533.

·         Ritter, J. B.; Genzel, Y.; Reichl, U.; "Monitoring of Extracellular TCA Cycle Intermediates in Mammalian Cell Culture " in Proceedings from the 19th ESACT meeting: Cell technology for cell products in June 2005, Harrogate, UK. Eds. R. Smith; Springer. 2007, 603-605.

·         Genzel, Y.; Schulze-Horsel, J.; Moehler, L.; Sidorenko, Y.; Reichl, U.; "Influenza Vaccines - Challenges in Mammalian Cell Culture Technology " in Proceedings from the 19th ESACT meeting: Cell technology for cell products in June 2005, Harrogate, UK. Eds. R. Smith; Springer. 2007, 503-508.

·         Ritter J.B.; Genzel Y.; Reichl U.; "High-performance anion-exchange chromatography using on-line electrolytic eluent generation for the determination of more than 25 intermediates from energy metabolism of mammalian cells in culture. " , J. Chrom. B , 2006, 843(2), 216-226.

·         Genzel Y.; Olmer R.M.; Schäfer B.; Reichl U.; "Wave microcarrier cultivation of MDCK cells for influenza virus production in serum containing and serum-free media. " , Vaccine , 2006, 24(35-36), 6074-6087.

·         Genzel Y.; Fischer M.; Reichl U.; "Serum-free influenza virus production avoiding washing steps and medium exchange in large-scale microcarrier culture. " , Vaccine , 2006, 24(16), 3261-3272.

·         Genzel Y.; Alt R.; Reichl U.; "Glutamine-free media for vaccine production processes" in Proceedings from the 18th ESACT meeting: Animal Cell Technology meets Genomics in May 2003, Granada, Spain.; Eds. F. Godia and M. Fussenegger; Springer. 2005, 767-770.

·         Genzel Y.; Ritter JB.; König S.; Alt R.; Reichl U.; "Substitution of glutamine by pyruvate to reduce ammonia formation and growth inhibition of mammalian cells." , Biotechnol. Progr. 2005, 21 (1), 58-69.

·         Genzel Y.; König S.; Reichl U.; "Amino acid analysis in mammalian cell culture media containing serum and high glucose concentrations by anion exchange chromatography and integrated pulsed amperometric detection (IPAD)." , Anal. Biochem. 2004, 335, 119-125.

·         Genzel Y.; Behrendt I.; König S.; Sann H.; Reichl U.; "Metabolism of MDCK cells during cell growth and influence virus production in large-scale microcarrier culture" , Vaccine 2004, 22(17-18), 2202-2208.

·         Reetz M.T.; Torre C.; Eipper A.; Lohmer R.; Hermes M.; Brunner B.; Maichele A.; Bocola M.; Arand M.; Cronin A.; Genzel Y.; Archelas A.; Furstoss R.; "Enhancing the enantioselectivity of an epoxide hydrolase by directed evolution" Org. Lett. 2004, 6(2), 177-180.

·         Genzel Y.; Reichl U.; "Analysis of Cell Growth, Virus Replication and Medium Composition in Equine Influenza Vaccine Production " , Chemie-Ingenieur-Technik 2002, 74(5), 673-674.

·         Genzel Y.; Archelas A.; Broxterman QB.; Schulze B.; Furstoss R.; "Microbiological transformations. 50: Selection of epoxide hydrolases for enzymatic resolution of 2-, -3- or -4-pyridyloxirane." J. Mol. Cat. B. Enzym. 2002, 16, 217-222.

·         Genzel Y.; Voges L.; Reichl U.; "Development of bioprocess concepts on vaccine production: influenza virus as an example" in Proceedings from the 17th ESACT meeting: Animal cell technology: From target to market in June 2001, Tylösand, Sweden.; Eds. E. Lindner-Olsson; N. Chatzissavidou; E. Luellau; Kluwer Acad. Publ..2001, 344-346.

·         Genzel Y.; Archelas A.; Lutje-Spelberg JH.; Janssen DB.; Furstoss R.; "Microbiological transformations. Part 48: Enantioselective biohydrolysis of 2-, 3- and 4-pyridyloxirane at high substrate concentration using the Agrobacterium radiobacter AD1 epoxide hydrolase and its Tyr215Phe mutant", Tetrahedron 2001, 57(14), 2775-2779.

·         Genzel Y.; Archelas A.; Broxterman QB.; Schulze B.; Furstoss R.; "Microbiological transformations. Part 47: A step toward a green chemistry preparation of enantiopure (S)-2-, -3- and -4-pyridyloxirane via an epoxide hydrolase catalyzed kinetic resolution." J. Org. Chem. 2001, 66(2), 538-543.

·         Genzel Y.; Archelas A.; Broxterman QB.; Schulze B.; Furstoss R.; "Microbiological transformations. Part 46: Preparation of enantiopure (S)-2-pyridyloxirane via epoxide hydrolase-catalyzed kinetic resolution." Tetrahedron: Asymmetry 2000, 11(15), 3041-3044.

·         Kroutil W.; Genzel Y.; Pietzsch M.; Syldatk C.; Faber K.; "Purification and characterization of a highly selective epoxide hydrolase from Nocardia sp. EH1." J. Biotechnol. 1998, 62(2), 143-150.

<genzel@mpi-magdeburg.mpg.de>

Last modified: 26/03/2012