Aktuelle Veröffentlichungen

Eric Schulze and Matthias Stein
Simulation of Mixed Self-Assembled Monolayers on Gold: Effect of Terminal Alkyl Anchor Chain and Monolayer Composition
The Journal of Physical Chemistry B, 122 (31), pp 7699–7710, published online 20 July 2018
DOI: 10.1021/acs.jpcb.8b05075

Peter Benner, Pawan Goyal, and Serkan Gugercin
H2-Quasi-Optimal Model Order Reduction for Quadratic-Bilinear Control Systems
SIAM Journal on Matrix Analysis and Applications, Vol. 39, Issue 2, pp. 983-1032, published online 5 June 2018
DOI: 10.1137/16M1098280

Thomas Beneyton, Dorothee Krafft, Claudia Bednarz, Christin Kleineberg, Christian Woelfer, Ivan Ivanov, Tanja Vidakovic-Koch, Kai Sundmacher und Jean-Christophe Baret
Out-of-equilibrium microcompartments for the bottom-up integration of metabolic functions
Nature Communications, 19 June 2018
DOI: 10.1038/s41467-018-04825-1

Petra Schwille, Joachim Spatz, Katharina Landfester, Eberhard Bodenschatz, Stephan Herminghaus,Victor Sourjik, Tobias Erb, Philippe Bastiaens, Reinhard Lipowsky, Anthony Hyman, Peter Dabrock, Jean-Christophe Baret, Tanja Vidakovic-Koch, Peter Bieling, Rumiana Dimova, Hannes Mutschler, Tom Robinson,Dora Tang, Seraphine Wegner, Kai Sundmacher
MaxSynBio ‐ Avenues towards creating cells from the bottom up
Angewandte Chemie, 11 May 2018
DOI: 10.1002/ange.201802288

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MPI Kolloquiumsreihe: Plant-Based Biopharmaceuticals: How to Move New Product Candidates Towards Translational Research and Market

The development of recombinant antibodies and vaccines has allowed us to treat and prevent a large number of life-threatening diseases. However, as things stand in 2015, the speed, capacity and scalability of current production systems is beginning to place limitations on this crucial technology. The large-scale production of antibodies, vaccines and other pharmaceutical recombinant proteins is restricted by the industry’s current reliance on fermenter technology, particularly the culture of mammalian cells. This expensive and time-consuming production platform is preventing the distribution of recombinant protein drugs to those most in need. One way in which the above limitations can be addressed is through the use of plants and plant-based expression systems for rapid recombinant pharmaceutical protein production. The economic production of plant-based pharmaceuticals depends on satisfactory yields and product quality. This presentation will discuss the latest development in antibody and vaccine development and their production by molecular farming, focusing particularly on strategies to maximize protein yields during upstream production and optimize protein recovery in the downstream processing steps. Such strategies often involve careful consideration of how the protein is expressed and targeted within the plant cell, a factor which affects yield, stability, quality and ease of isolation. Our long-term objective is to ensure that next generation of plantbased production systems for recombinant proteins will create the opportunity to deliver antibodies, vaccines and other biopharmaceuticals beyond the industrialized nations and into the developing world. Several case studies will be presented: HIV antibodies were chosen to undergo fast-track development, including risk assessment, expression in tobacco, scale-up, downstream processing and regulatory development, with the aim of performing clinical trials. In addition use of engineered plant cells for human vaccine candidates will be discussed. Pharma-Planta is an EU Sixth Framework Integrated Project whose primary goal is to develop an approved production pipeline for plant-derived pharmaceutical proteins (PDPs). Although previous research has provided proof of the PDP concept, Pharma-Planta aims to develop an entire production chain by taking candidate pharmaceutical molecules from the expression platform through all stages of production and processing, ultimately to initiate phase I human trials in Europe. The Pharma-Planta Consortium comprised 40 interacting groups representing 33 public institutes and SMEs from 11 European Member States and South Africa. At the beginning of the project, eight target molecules were chosen representing four key indication areas including HIV. From these molecules, two HIV antibodies were chosen to undergo fast-track development, which would include risk assessment, cloning, expression and optimization of production in plants, scale-up, downstream processing and regulatory development, with the aim of submitting at least one of them for clinical trials within the five years of the program. Two HIV neutralizing antibodies have been expressed successfully in the two main production crops being developed within the consortium – maize and tobacco. One of these antibodies, 2G12, has been expressed at levels greater 100 mg per kg of plant material. The plant-derived antibodies remain stable and functional and retain their neutralizing activity. The consortium has investigated novel upscaling and downstream processing strategies to provide multiple grams clinical grade antibody material for human clinical trials. Preclinical trials in rabbits have been completed successfully and we also conducted successfully a phase I clinical trial in the UK. This work will now be moved forward for a phase IIa clinical trial which is funded by an Advanced ERC grant from the European Commission. We have also developed an interesting multi-stage malaria vaccine and neutralizing rabies antibody candidates and will discuss how both products have matured over the years both in performance and in manufacturing with the aim in mind to bring these two products into translational research within the next months. Finally state of the art technology developments to accelerate the development and production of PMPs as well as regulatory issues will be discussed. Along this line an innovative new manufacturing concept using LED lighting in a vertical farm concept have been developed. [mehr]

ENTFÄLLT: MPI Kolloquiumsreihe: Prof. Dr. Rainer Fischer

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ENTFÄLLT: MPI Kolloquiumsreihe: Prof. Dr. Rainer Fischer


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