Head of the Group

Prof. Dr.-Ing. Udo Reichl
Prof. Dr.-Ing. Udo Reichl
Phone: +49 391 6110 201
Fax: +49 391 6110 203
Room: N0.19

Contact Person

Annegret Frauendienst
Phone: +49 391 6110 200
Fax: +49 391 6110 203
Room: N0.20

Group Page

Publications

Publications BPE group

Journal Article (206)

  1. 2010
    Öncül, A.; Kalmbach, A.; Genzel, Y.; Reichl, U.; Thevenin, D.: Characterization of flow conditions in 2 L and 20 L wave bioreactors using Computational Fluid Dynamics. Biotechnology Progress 26 (1), pp. 101 - 110 (2010)
  2. 2009
    Bock, A.; Möhler, L.; Sann, H.; Schulze-Horsel, J.; Genzel, Y.; Reichl, U.: Growth behaviour of number distributed adherent MDCK cells for optimization in large scale microcarrier cultures. Biotechnology Progress 25 (6), pp. 1717 - 1731 (2009)
  3. Genzel, Y.; Reichl, U.: Continuous cell lines as production system for influenza vaccines. Expert Review of Vaccines 8 (12), pp. 1681 - 1692 (2009)
  4. Lohr, V.; Rath, A.; Genzel, Y.; Jordan, I.; Sandig, V.; Reichl, U.: New avian suspension cell lines provide production of influenza virus and MVA in serum-free media: studies on growth, metabolism and virus propagation. Vaccine 27 (36), pp. 4975 - 4982 (2009)
  5. Opitz, L.; Lehmann, S.; Reichl, U.; Wolff, M. W.: Sulfated membrane adsorbers for economic pseudo-affinity capture of influenza virus particles. Biotechnology and Bioengineering 103 (6), pp. 1144 - 1154 (2009)
  6. Opitz, L.; Hohlweg, J.; Reichl, U.; Wolff, M. W.: Purification of cell culture-derived influenza virus A/Puerto Rico/8/34 by membrane-based immobilized metal affinity chromatography. Journal of Virological Methods 161 (2), pp. 312 - 316 (2009)
  7. Rapp, E.; Charvat, A.; Beinsen, A.; Plessmann, U.; Reichl, U.; Seidel-Morgenstern, A.; Urlaub, H.; Abel, B.: Atmospheric Pressure Free Liquid Infrared MALDI Mass Spectrometry: Toward a combined ESI/MALDI-Liquid Chromatography Interface. Analytical Chemistry 81 (1), pp. 443 - 452 (2009)
  8. Schulze-Horsel, J.; Schulze, M.; Agalaridis, G.; Genzel, Y.; Reichl, U.: Infection dynamics and virus-induced apoptosis in cell culture-based influenza vaccine production – flow cytometry and mathematical modeling. Vaccine 27 (20), pp. 2712 - 2722 (2009)
  9. Schwarzer, J.; Rapp, E.; Hennig, R.; Genzel, Y.; Jordan, I.; Sandig, V.; Reichl, U.: Glycan analysis in cell culture-based influenza vaccine production: Influence of host cell line and virus strain on the glycosylation pattern of viral hemagglutinin. Vaccine 27 (32), pp. 4325 - 4336 (2009)
  10. Vester, D.; Rapp, E.; Gade, D.; Genzel, Y.; Reichl, U.: Quantitative analysis of cellular proteome alterations in human influenza A virus-infected mammalian cell lines. Proteomics 9 (12), pp. 3316 - 3327 (2009)
  11. 2008
    Antoniukas, L.; Grammel, H.; Sasnauskas, K.; Reichl, U.: Profiling of external metabolites during production of hantavirus nucleocapsid protein with recombinant Saccharomyces cerevisiae. Biotechnology Letters 30 (3), pp. 415 - 420 (2008)
  12. Kalbfuss, B.; Knöchlein, A.; Kröber, T.; Reichl, U.: Monitoring Influenza Virus Content in Vaccine Production: Precise Assays for the Quantitation of Hemagglutination and Neuraminidase Activity. Biologicals 36 (3), pp. 145 - 161 (2008)
  13. Kalbfuss, B.; Flockerzi, D.; Seidel-Morgenstern, A.; Reichl, U.: Size-exclusion chromatography as a linear transfer system: Purification of human influenza virus as an example. Journal of Chromatography B 873 (1), pp. 102 - 112 (2008)
  14. Möhler, L.; Bock, A.; Reichl, U.: Segregated Mathematical Model for Growth of Anchorage-Dependent MDCK Cells in Microcarrier Culture. Biotechnology Progress 24 (1), pp. 110 - 119 (2008)
  15. Opitz, L.; Zimmermann, A.; Lehmann, S.; Genzel, Y.; Lübben, H.; Reichl, U.; Wolff, M. W.: Capture of cell culture-derived influenza virus by lectins: strain independent, but host cell dependent. Journal of Virological Methods 154 (1-2), pp. 61 - 68 (2008)
  16. Pohlscheidt, M.; Kauling, D. J.; Havekost, D.; Bödeker, B.; Langer, U.; Apeler, H.; Hörlein, H. D.; Rübsamen-Waigmann, H.; Reichl, U.; Henzler, H. J.: Auslegungsunterlagen für Bioreaktoren zur Maßstabsvergrößerung eines microcarrierbasierenden Virusproduktionsprozesses. Chemie-Ingenieur-Technik 80 (6), pp. 821 - 830 (2008)
  17. Ritter, J. B.; Genzel, Y.; Reichl, U.: Simultaneous extraction of several metabolites of energy metabolism and related substances in mammalian cells: Optimization using experimental design. Analytical Biochemistry 373, pp. 349 - 369 (2008)
  18. Schulze-Horsel, J.; Genzel, Y.; Reichl, U.: Flow cytometric monitoring of influenza A virus infection in MDCK cells during vaccine production. BMC Biotechnology 8, p. 45 (2008)
  19. Schwarzer, J.; Rapp, E.; Reichl, U.: N-Glycan Analysis by CGE-LIF – Profiling Influenza A Virus Hemagglutinin N-Glycosylation during Vaccine Production. Electrophoresis 29 (20), pp. 4203 - 4214 (2008)
  20. Sidorenko, Y.; Schulze-Horsel, J.; Voigt, A.; Reichl, U.; Kienle, A.: Stochastic Population Balance Modeling of Influenza Virus Replication in Vaccine Production Processes. Chemical Engineering Science 63, pp. 157 - 169 (2008)
  21. Sidorenko, Y.; Wahl, A.; Dauner, M.; Genzel, Y.; Reichl, U.: Comparison of Metabolic Flux Distributions for MDCK Cell Growth in Glutamine- and Pyruvate-Containing Media. Biotechnology Progress 24 (2), pp. 311 - 320 (2008)
  22. Sidorenko, Y.; Antoniukas, L.; Schulze-Horsel, J.; Reichl, U.: Mathematical model of growth and heterologous hantavirus protein production of the recombinant yeast Saccharomyces cerevisiae. Engineering in Life Sciences 8 (4), pp. 399 - 414 (2008)
  23. Sidorenko, Y.; Voigt, A.; Schulze-Horsel, J.; Reichl, U.; Kienle, A.: Stochastic population balance modeling of influenza virus replication in vaccine production processes. II. Detailed description of the replication mechanism. Chemical Engineering Science 63 (8), pp. 2299 - 2304 (2008)
  24. Wahl, A.; Sidorenko, Y.; Dauner, M.; Genzel, Y.; Reichl, U.: Metabolic flux model for an anchorage-dependent MDCK cell line: Characteristic Growth Phases and Minimum Substrate Consumption Flux Distribution. Biotechnology and Bioengineering 101 (1), pp. 135 - 152 (2008)
  25. Wahl, S. A.; Nöh, K.; Wiechert, W.: 13C labeling experiments at metabolic nonstationary conditions: an exploratory study. BMC Bioinformatics 9, p. 152 (2008)
  26. Wolff, M. W.; Reichl, U.: Downstream Processing: From egg to cell culture-derived influenza virus particles. Chemical Engineering and Technology 31 (6), pp. 846 - 857 (2008)
  27. 2007
    Bandaru, K. S.V.S.R.; Keßler, L. C.; Wolff, M. W.; Reichl, U.; Seidel-Morgenstern, A.; Pushpavanam, S.: Hydrodynamic characteristics and expansion behavior of beds containing single and binary-mixtures of particles. Industrial and Engineering Chemistry Research 46 (13), pp. 4686 - 4694 (2007)
  28. Hundt, B.; Best, C.; Schlawin, N.; Kassner, H.; Genzel, Y.; Reichl, U.: Establishment of a mink enteritis vaccine production process in stirred-tank reactor and Wave Bioreactor microcarrier culture in 1-10 L scale. Vaccine 25 (20), pp. 3987 - 3995 (2007)
  29. Kalbfuss, B.; Genzel, Y.; Wolff, M. W.; Zimmermann, A.; Morenweiser, R.; Reichl, U.: Harvesting and concentration of human influenza A virus produced in serum-free mammalian cell culture for the production of vaccines. Biotechnology and Bioengineering 97 (1), pp. 73 - 85 (2007)
  30. Kalbfuss, B.; Wolff, M. W.; Morenweiser, R.; Reichl, U.: Purification of cell culture-derived human influenza A virus by size-exclusion and anion-exchange chromatography. Biotechnology and Bioengineering 96, pp. 932 - 944 (2007)
  31. Kalbfuss, B.; Wolff, M. W.; Geisler, L.; Tappe, A.; Wickramasinghe, R.; Thom, V.; Reichl, U.: Direct capture of influenza A virus from cell culture supernatant with Sartobind anion-exchange membrane adsorbers. Journal of Membrane Science 299, pp. 251 - 260 (2007)
  32. Nischang, I.; Reichl, U.; Seidel-Morgenstern, A.; Tallarek, U.: Concentration Polarization and Nonequilibrium Electroosmotic Slip in Dense Multiparticle Systems. Langmuir 23 (18), pp. 9271 - 9281 (2007)
  33. Noack, S.; Wahl, A.; Qeli, E.; Wiechert, W.: Visualizing Regulatory Interactions in Metabolic Networks. BMC Biology 5, p. 46 (2007)
  34. Opitz, L.; Salaklang, J.; Büttner, H.; Reichl, U.; Wolff, M. W.: Lectin-affinity chromatography for downstream processing of MDCK cell culture derived human influenza A viruses. Vaccine 25 (5), pp. 939 - 947 (2007)
  35. Opitz, L.; Lehmann, S.; Zimmermann, A.; Reichl, U.; Wolff, M. W.: Impact of adsorbents selection on affinity purification efficiency of cell culture derived human Influenza viruses. Journal of Biotechnology 131 (3), pp. 309 - 317 (2007)
  36. Pohlscheidt, M.; Langer, U.; Minuth, T.; Bödeker, B.; Apeler, H.; Hörlein, H. D.; Paulsen, D.; Rübsamen-Waigmann, H.; Henzler, H. J.; Reichl, U.: Development and optimisation of a procedure for the production of Parapoxvirus Ovis by large scale microcarrier cell culture in a non animal, non human and non plant derived medium. Vaccine 26 (12), pp. 1552 - 1565 (2007)
  37. 2006
    Antoniukas, L.; Grammel, H.; Reichl, U.: Production of hantavirus Puumala nucleocapsid protein in Saccharomyces cerevisiae for vaccine and diagnostics. Journal of Biotechnology 124 (2), pp. 347 - 362 (2006)
  38. Genzel, Y.; Fischer, M.; Reichl, U.: Serum-free influenza virus production avoiding washing steps & medium exchange in large-scale microcarrier culture. Vaccine 24, pp. 3261 - 3272 (2006)
  39. Genzel, Y.; Olmer, R. M.; Schäfer, B.; Reichl, U.: Wave microcarrier cultivation of MDCK cells for influenza virus production in serum containing and serum-free media. Vaccine 24 (35-36), pp. 6074 - 6087 (2006)
  40. Heßeler, J.; Schmidt, J. K.; Reichl, U.; Flockerzi, D.: Coexistence in the Chemostat as a result of metabolic by-products. Journal of mathematical biology 53 (4), pp. 556 - 584 (2006)
  41. Kalbfuss, B.; Reichl, U.: Preparative Size-Exclusion Chromatography. GIT Laboratory Journal Europe 10 (4), pp. 18 - 20 (2006)
  42. Ritter, J. B.; Genzel, Y.; Reichl, U.: High-performance anion-exchange chromatography using on-line electrolytic eluent generation for the determination of more than 25 intermediates from energy metabolism of mammalian cells in culture. Journal of Chromatography B 843 (2), pp. 216 - 226 (2006)
  43. Schmidt, J. K.; König, B.; Reichl, U.: Characterization of a Three Bacteria Mixed Culture in a Chemostat: Evaluation and Application of a Quantitative Terminal-Restriction Fragment Polymorphism (T-RFLP) Analysis for Absolute and Species Specific Cell Enumeration. Biotechnology and Bioengineering 96 (4), pp. 738 - 756 (2006)
  44. 2005
    Genzel, Y.; Ritter, J. B.; König, S.; Alt, R.; Reichl, U.: Substitution of glutamine by pyruvate to reduce ammonia formation and growth inhibition of mammalian cells. Biotechnology Progress 21 (1), pp. 58 - 69 (2005)
  45. Möhler, L.; Flockerzi, D.; Sann, H.; Reichl, U.: Mathematical Model of Influenza A Virus Production in Large-Scale Microcarrier Culture. Biotechnology and Bioengineering 90 (1), pp. 46 - 58 (2005)
  46. Nayak, D. P.; Lehmann, S.; Reichl, U.: Downstream processing of MDCK cell-derived equine influenza virus. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 823 (2), pp. 75 - 81 (2005)
  47. Wickramasinghe, S. R.; Kalbfuss, B.; Zimmermann, A.; Thom, V.; Reichl, U.: Tangential flow microfiltration and ultrafiltration for human influenza A virus concentration and purification. Biotechnology and Bioengineering 92 (2), pp. 199 - 208 (2005)
  48. 2004
    Genzel, Y.; König, S.; Reichl, U.: Amino acid analysis in mammalian cell culture media containing serum and high glucose concentrations by anion exchange chromatography and integrated pulsed amperometric detection. Analytical Biochemistry 335, pp. 119 - 125 (2004)
  49. Genzel, Y.; Behrendt, I.; König, S.; Sann, H.; Reichl, U.: Metabolism of MDCK Cells during cell growth and influenza virus production in large-scale microcarrier culture. Vaccine 22, pp. 2202 - 2208 (2004)
  50. Genzel, Y.; Reichl, U.: Bioprozesstechnik und Impfstoff-Forschung an der Elbe. Bioforum 27 (5), pp. 44 - 46 (2004)
 
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