Team Leader (USP)

PD Dr. Yvonne Genzel
PD Dr. Yvonne Genzel
Phone: +49 391 6110 257
Room: N0.18

Researcher

Dipl.-Biol. (t.o.) Thomas Bissinger
Dipl.-Biol. (t.o.) Thomas Bissinger
Phone: +49 391 6110 131
Room: N 0.07

Quantitative Analysis of Metabolic and Regulatory Networks of Cellular Systems

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Quantitative Analysis of Metabolic and Regulatory Networks of Cellular Systems

For a better understanding of cell growth and product formation in animal cell culture, comprehensive quantitative data sets are collected over cultivation processes [1-3]. These data can give insight into the morphology, growth state and metabolism of the cultivated cell population. With the help of mathematical models, the data can be used to support quantitative analysis, optimization, and process evaluation [4, 5].

Experimental data considered:

  • Cell physiology [4]
    Cell size, cell count, cell cycle, apoptosis
  • Extracellular metabolites [3]
    Substrates (pyruvate, glucose, amino acids), inhibitors (lactate, ammonia)
  • Intracellular metabolites [2]
    Nucleotides, glycolytic intermediates, TCA intermediates
  • Enzyme activities [1]
    intracellular enzyme activity
<em><strong>Figure:</strong> Extracellular (orange and yellow) and intracellular (red) metabolites comprise together with enzymes the metabolic cell network.</em> Zoom Image
Figure: Extracellular (orange and yellow) and intracellular (red) metabolites comprise together with enzymes the metabolic cell network. [less]

References

1.
Janke, R.; Genzel, Y.; Wahl, A.; Reichl, U.: Measurement of Key Metabolic Enzyme Activities in Mammalian Cells Using Rapid and Sensitive Microplate-Based Assays. Biotechnology and Bioengineering 107 (3), pp. 566 - 581 (2010)
2.
Ritter, J. B.; Genzel, Y.; Reichl, U.: High-performance anion-exchange chromatography using on-line electrolytic eluent generation for the determination of more than 25 intermediates from energy metabolism of mammalian cells in culture. Journal of Chromatography B 843 (2), pp. 216 - 226 (2006)
3.
Genzel, Y.; König, S.; Reichl, U.: Amino acid analysis in mammalian cell culture media containing serum and high glucose concentrations by anion exchange chromatography and integrated pulsed amperometric detection. Analytical Biochemistry 335, pp. 119 - 125 (2004)
4.
Rehberg, M.; Ritter, J. B.; Genzel, Y.; Flockerzi, D.; Reichl, U.: The relation between growth phases, cell volume changes and metabolism of adherent cells during cultivation. Journal of Biotechnology 164 (4), pp. 489 - 499 (2013)
5.
Rehberg, M.; Ritter, J.; Reichl, U.: Glycolysis Is Governed by Growth Regime and Simple Enzyme Regulation in Adherent MDCK Cells. PLoS Computational Biology 10 (10), p. e1003885 (2014)
 
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