Team Leader (BPA)

Dr. Erdmann Rapp
Dr. Erdmann Rapp
Phone: +49 391 6110 314
Fax: +49 391 6110 535
Room: S0.13

Researchers

Dipl.-Ing. Marcus Hoffmann
Phone: +49 391 6110 427
Room: S0.07
Sebastian Püttker, M. Sc.
Sebastian Püttker, M. Sc.
Phone: +49 391 67570 69
Room: G28-300

Qualitative and Quantitative Analysis of Biotechnological Systems on Proteome Level

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Qualitative and Quantitative Analysis of Biotechnological Systems on Proteome Level

Proteome Analysis in Cell Culture-Based Vaccine Production

Using qualitative and quantitative proteomic profiling, basic insights into cellular pathways involved in virus replication in cell lines used for vaccine production can be obtained. In particular, the impact of virus-induced changes in cellular processes related to apoptosis and stress response is being characterized. Furthermore, hints on proteins and cellular pathways involved in intracellular virus replication are obtained that provide insight into the complexity and dynamics of virus-host cell interactions. The findings will improve understanding of host cell response mechanisms relevant in vaccine production and support optimization of cell culture-based vaccine manufacturing processes. In addition, progress in understanding viral strategies to evade host cell responses and to replicate efficiently in different cell lines may help to accelerate the identification of antiviral compounds.

Figure 1: Qualitative and quantitative proteomic profiling is used to get basic insights into cellular pathways involved in influenza virus replication in cell lines used for vaccine production on the proteome level. Zoom Image
Figure 1: Qualitative and quantitative proteomic profiling is used to get basic insights into cellular pathways involved in influenza virus replication in cell lines used for vaccine production on the proteome level. [less]

Proteome Analysis of Bacteria

Qualitative and quantitative proteomic profiling can also be used to investigate microbial response to environmental changes, interspecies interactions and growth behavior of microbial communities. Analysis is performed using a set of techniques based on gel-electrophoresis protein separation and quantification. In addition, off-gel techniques in combination with two-dimensional high performance liquid chromatography coupled online and offline to tandem mass spectrometry are used for protein identification.

 
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