Qualitative and Quantitative Analysis of Biotechnological Systems on Proteome Level
Proteome Analysis in Cell Culture-Based Vaccine Production
Using qualitative and quantitative proteomic profiling, basic insights into cellular pathways involved in virus replication in cell lines used for vaccine production can be obtained. In particular, the impact of virus-induced changes in cellular processes related to apoptosis and stress response is being characterized. Furthermore, hints on proteins and cellular pathways involved in intracellular virus replication are obtained that provide insight into the complexity and dynamics of virus-host cell interactions. The findings will improve understanding of host cell response mechanisms relevant in vaccine production and support optimization of cell culture-based vaccine manufacturing processes. In addition, progress in understanding viral strategies to evade host cell responses and to replicate efficiently in different cell lines may help to accelerate the identification of antiviral compounds.
Proteome Analysis of Bacteria
Qualitative and quantitative proteomic profiling can also be used to investigate microbial response to environmental changes, interspecies interactions and growth behavior of microbial communities. Analysis is performed using a set of techniques based on gel-electrophoresis protein separation and quantification. In addition, off-gel techniques in combination with two-dimensional high performance liquid chromatography coupled online and offline to tandem mass spectrometry are used for protein identification.