Programme

Programme

Day 1

8:15

Registration, Check-In, Coffee&Tea

9:15

Welcome and opening remarks

9:30

Keynote Lecture:

Markus Aebi, ETH Zurich, Switzerland 

Freedom to operate: concepts of N-linked protein glycosylation

 

Session: Glycosylation & Protein Function

Chair: Niclas Karlsson and Rita Gerardy-Schahn

10:10

Elisa Benedetti, Helmholtz Center Munich, Germany

Network inference from glycoproteomics data reveals new reactions in the IgG glycosylation pathway

10:30

Gordan Lauc, University of Zagreb, Croatia

Functional relevance of inter-individual differences in the IgG glycome

10:50

Anna-Janina Behrens, University of Oxford, United Kingdom

Site-specific N-glycosylation analysis of the HIV-1 glycan shield

11:10

Break – Coffee&Tea, Pastries, Fruits

11:40

Serge Perez, CERMAV-CNRS, University of Grenoble Alpes, France

Dynamics of fucose and Lewis x in lectin-binding sites

12:00

Katrine Schjoldager, Copenhagen Center for Glycomics, Denmark

Site-specific O-glycosylation modulates HDL-metabolism in vivo

12:20

Jens Kopatz, University Hospital Bonn, Germany

Low molecular weight polysialic acid inhibits the innate immune system and is a promising candidate for therapy of age related macular degeneration

12:40

Rob Field, John Innes Centre, United Kingdom

Dissecting plant polysaccharide biosynthesis

13:00

Lunch Break

14:15

Invited Lecture:

Dietmar Reusch, F. Hoffmann - La Roche AG, Germany 

Comparison of methods for IgG glycosylation - a multi laboratory study

 

Session: Technologies & Methods for Glycoanalysis

Chair: Erdmann Rapp

14:50

Alexandra Stuckmann, Free University Berlin, Germany

Ion mobility-mass spectrometry of isomeric glycans – the impact of reducing end modifications

15:10

Kathrin Stavenhagen, VU University Amsterdam, The Netherlands

Site-specific N- and O-glycosylation analysis of antibody-based therapeutics

15:30

Monique van Scherpenzeel, Radboudumc, The Netherlands

Sensitive ion pair UHPLC-QqQ to analyze the building blocks of glycosylation gives new mechanistic insights and opportunities for therapy supplementation in congenital disorders of glycosylation

15:50

Break – Coffee&Tea, Pastries, Fruits

16:20

Radoslaw Kozak, Ludger Ltd., United Kingdom

Analysis of biopharmaceutical Glycosylation Critical Quality Attributes (GCQAs)

16:40

Jean Dubayle, Sanofi Pasteur, France

Advances in glycosylation characterization of vaccine antigens

17:00

Mario Schubert, University Salzburg, Austria

Characterizing glycans in intact proteins by NMR spectroscopy

17:20

Jun Hirabayashi, AIST, Japan

Development of lectin-based glycan profiling technologies: reinforced frontal affinity chromatography and evanescent wave-activated lectin microarray

17:40

Falk Buettner, Hannover Medical School, Germany

Glycoproteomic analysis of human pluripotent stem cells and stem cell-derived cardiomyocytes

18:00

Dine&Discuss – Food, beer, wine and soft drinks are included

 

 

Day 2

9:00

Keynote Lecture:

Nico Callewaert, VIB Medical Biotechnology Center, Belgium

Customization of biopharmaceutical protein glycosylation

 

Session: Glycoengineering via Cell Line Design

Chair: Nathan Lewis and Alexander Frey

9:40

Nathan Lewis, University of California, San Diego, United States

A systems approach to cell line selection and design for rational glycoengineering

10:00

Alexander Frey, Aalto University, Finland

Impact of glycoengineering on glycosylation efficiency, cellular quality control systems and product quality

10:20

Robert Woods, CCRC, University of Georgia, United States

Predicting N-glycan processing based on enzyme-glycan accessibility

10:40

Break – Coffee&Tea, Pastries, Fruits

11:10

Invited Lecture:

Henrik Clausen, Copenhagen Center for Glycomics, Denmark

Stable glycoengineering of mammalian cells

11:45

Niklas Karlsson, University of Gothenburg, Sweden

Glycomodified Chinese Hamster Ovary cells to investigate glycosyltransferase specificity and function of O-linked glycans

12:05

Stephan Hinderlich, Beuth University of Applied Sciences Berlin, Germany

Engineering of CHO cells for the production of recombinant glycoproteins with xylosylated N-glycans

12:25

Lennard Bohlender, University of Freiburg, Germany

Production of biologically active recombinant factor H in glyco-optimized Physcomitrella patens

12:45

Lunch Break

14:05

Sabine Flitsch, University of Manchester, United Kingdom

Metrology of carbohydrates for enabling European bioIndustries

14:10

Keynote Lecture:

Michael J. Betenbaugh, John Hopkins University, United States

Looking inside and out: Systems approaches to glycoengineering of mammalian bioprocesses

 

Session: Synthetic & Biosynthetic Glycoengineering

Chair: Lothar Elling and Markus Pietzsch

14:50

Marco Thomann, Roche Diagnostics GmbH, Germany

In vitro glycoengineering – A useful tool for protein characterization and optimization through modification of the glycosylation pattern

15:10

Lothar Elling, Helmholtz Institute for Biomedical Engineering RWTH Aachen, Germany

Multivalent neo-glycoproteins as specific galectin ligands and serum diagnostics

15:30

Anna Schildbach, MLU Halle-Wittenberg, Germany

In-vitro synthesis of phytanyl-pyrophosphate-linked oligosaccharides by cascades of glycosyltransferases as basic platform of N-glycosylation of therapeutic proteins

15:50

Break – Coffee&Tea, Pastries, Fruits

16:20

Jonathan Sjörgen, Genovis AB, Sweden

Site specific and quantitative conjugation of functional groups to antibody N-glycans using the novel GlyCLICK™ technology

16:40

Peng George Wang, Georgia State University, United States

Enzymatic synthesis and bioengineering for synthetic glycomes

17:00

Timm Fiebig, Medical School Hannover, Germany

An enzyme-based synthesis of a meningococcal glycoconjugate vaccine

17:20

Anne Imberty, CERMAV-CNRS, University of Grenoble Alpes, France

New tools from synthetic glycobiology: Engineering lectins valency, architecture and specificity

17:40

Daniel Varon Silva, Max Planck Institute of Colloids and Interfaces, Germany

Protein semi-synthesis as tool to disclose the role of protein glypiation

18:00

Dine&Discuss – Food, beer, wine and soft drinks are included

 

 

Day 3

 

9:00

Invited Lecture:

Michael Butler, NIBRT, Ireland

The influence of cultivation conditions on glycosylation

 

Session: Impact of Expression Systems & Cultivation Conditions on Glycosylation

Chair: Udo Reichl

9:35

David Bruehlmann, Merck Biopharma, Switzerland

The Potential of small molecules to modulate glycosylation by media design

9:55

Lars Stoeckl, Glycotope GmbH, Germany

A toolbox of human cell lines for the screening and production of fully human and glycooptimized biotherapeutics

10:15

Break – Coffee&Tea, Pastries, Fruits

10:35

Ioscani Jimenez del Val, University College Dublin, Ireland

A metabolic flux model that quantifies the partition of resources between cellular and recombinant protein glycosylation in GS-CHO cells

10:55

Stefan Kubick, Fraunhofer Institute for Cell Therapy and Immunology, Germany

Cell-free synthesis of glycoproteins

11:15

Closing remarks

11:30

End of the symposium

 

 

 
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